Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method

Ashok K. Singh; Usha Mishra; Muhammed Ashraf; El Hassane Abdennebi; Kae Granley; Dzintra Dombrovskis; Dave Hewetson; Clarence M. Stowe

doi:10.1016/S0021-9673(01)86852-7

Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method

Ashok K. Singh
, Usha Mishra
, Muhammed Ashraf
, El Hassane Abdennebi
, Kae Granley
, Dzintra Dombrovskis
, Dave Hewetson
, Clarence M. Stowe

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Chemical ionization- an electron impact ionization-selective ion monitoring provided a simple and sensitive method for measuring detomidine (Domosedan), a potent sedative-analgesic drug for horses and cattle. Chemical ionization was at least 10 times more sensitive than electron impact ionization. By using propranolol as an internal standard, we found that the recovery of detomidine from the extraction procedure used in this study was greater than 75% for plasma, whole blood, or urine samples. Approximately 68% of detomidine was bound to plasma protein and 53% was bound to red blood cells.

Original language	English (US)
Pages (from-to)	223-232
Number of pages	10
Journal	Journal of Chromatography A
Volume	404
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(01)86852-7
State	Published - 1987

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title = "Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method",

abstract = "Chemical ionization- an electron impact ionization-selective ion monitoring provided a simple and sensitive method for measuring detomidine (Domosedan), a potent sedative-analgesic drug for horses and cattle. Chemical ionization was at least 10 times more sensitive than electron impact ionization. By using propranolol as an internal standard, we found that the recovery of detomidine from the extraction procedure used in this study was greater than 75\% for plasma, whole blood, or urine samples. Approximately 68\% of detomidine was bound to plasma protein and 53\% was bound to red blood cells.",

author = "Singh, \{Ashok K.\} and Usha Mishra and Muhammed Ashraf and Abdennebi, \{El Hassane\} and Kae Granley and Dzintra Dombrovskis and Dave Hewetson and Stowe, \{Clarence M.\}",

year = "1987",

doi = "10.1016/S0021-9673(01)86852-7",

language = "English (US)",

volume = "404",

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journal = "Journal of Chromatography A",

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T1 - Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method

AU - Singh, Ashok K.

AU - Mishra, Usha

AU - Ashraf, Muhammed

AU - Abdennebi, El Hassane

AU - Granley, Kae

AU - Dombrovskis, Dzintra

AU - Hewetson, Dave

AU - Stowe, Clarence M.

PY - 1987

Y1 - 1987

N2 - Chemical ionization- an electron impact ionization-selective ion monitoring provided a simple and sensitive method for measuring detomidine (Domosedan), a potent sedative-analgesic drug for horses and cattle. Chemical ionization was at least 10 times more sensitive than electron impact ionization. By using propranolol as an internal standard, we found that the recovery of detomidine from the extraction procedure used in this study was greater than 75% for plasma, whole blood, or urine samples. Approximately 68% of detomidine was bound to plasma protein and 53% was bound to red blood cells.

AB - Chemical ionization- an electron impact ionization-selective ion monitoring provided a simple and sensitive method for measuring detomidine (Domosedan), a potent sedative-analgesic drug for horses and cattle. Chemical ionization was at least 10 times more sensitive than electron impact ionization. By using propranolol as an internal standard, we found that the recovery of detomidine from the extraction procedure used in this study was greater than 75% for plasma, whole blood, or urine samples. Approximately 68% of detomidine was bound to plasma protein and 53% was bound to red blood cells.

UR - https://www.scopus.com/pages/publications/0023212693

UR - https://www.scopus.com/pages/publications/0023212693#tab=citedBy

U2 - 10.1016/S0021-9673(01)86852-7

DO - 10.1016/S0021-9673(01)86852-7

M3 - Article

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AN - SCOPUS:0023212693

SN - 0021-9673

VL - 404

SP - 223

EP - 232

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - C

ER -

Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method

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