Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method

Ashok K. Singh, Usha Mishra, Muhammed Ashraf, El Hassane Abdennebi, Kae Granley, Dzintra Dombrovskis, Dave Hewetson, Clarence M. Stowe

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Chemical ionization- an electron impact ionization-selective ion monitoring provided a simple and sensitive method for measuring detomidine (Domosedan), a potent sedative-analgesic drug for horses and cattle. Chemical ionization was at least 10 times more sensitive than electron impact ionization. By using propranolol as an internal standard, we found that the recovery of detomidine from the extraction procedure used in this study was greater than 75% for plasma, whole blood, or urine samples. Approximately 68% of detomidine was bound to plasma protein and 53% was bound to red blood cells.

Original languageEnglish (US)
Pages (from-to)223-232
Number of pages10
JournalJournal of Chromatography A
Volume404
Issue numberC
DOIs
StatePublished - 1987

Fingerprint Dive into the research topics of 'Analysis of detomidine in horse blood, plasma and urine samples utilizing a sensitive gas chromatography-mass spectrometry method'. Together they form a unique fingerprint.

Cite this