Analysis of Crassostrea gasar transcriptome reveals candidate genes involved in metal metabolism

Clarissa P. Ferreira, Renato S. Moreira, Guilherme Toledo-Silva, Declan C. Schroeder, Afonso C.D. Bainy, Karim H. Lüchmann

Research output: Contribution to journalArticlepeer-review

Abstract

Oysters have been extensively employed for monitoring of metal pollution in dynamic aquatic ecosystems. Therefore, the use of specific biomarkers can assist in discriminating the ecotoxicological implications of different elements in such complex environments. In this study, we revisited the sequencing data of gills and digestive glands transcripts in the mangrove oyster Crassostrea gasar and generated a reference transcriptome assembly from multiple assemblers, seven in total. Overall, we were able to identify a total of 11,917 transcripts, with 86.6% of them being functionally annotated and 1.4 times more than the first annotation. We screened the annotated transcripts to identify genes potentially involved in metals' transport, storage, and detoxification. Our findings included genes related to Zn distribution in cells (Zn transporters - ZIP, ZnT), metallothionein (MT-I and MT-IV), GSH biosynthesis, Ca+ transporter (NCX and ATP2B), and Cu distribution in cells (ATP7, ATOX1, CCS, and laccase-like). These results provided a reference transcriptome for additional insights into the transcriptional profile of C. gasar and other bivalves to better understand the molecular pathways underpinning metal tolerance and susceptibility. The study also provided an auxiliary tool for biomonitoring metal contamination in dynamic environments as estuaries.

Original languageEnglish (US)
Article number136009
JournalChemosphere
Volume307
DOIs
StatePublished - Nov 2022

Bibliographical note

Funding Information:
CPF was supported by the fellowship of Postgraduate Monitoring Scholarship Program (PROMOP) from Santa Catarina State University (UDESC), Brazil. CPF was a Guest Student at the Veterinary Population Medicine Department at the University of Minnesota, and the scholarship was supported by the Doctoral Sandwich Abroad Program (PDSE/CAPES, 19/2020). A.C.D. Bainy is recipient of the CNPq productivity fellowship.

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

  • Biomarkers
  • Metal-metabolism
  • Oysters
  • Sentinel organisms

PubMed: MeSH publication types

  • Journal Article

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