Analysis of crab meat volatiles as possible spoilage indicators for blue crab (Callinectes sapidus) meat by gas chromatography-mass spectrometry

Paul J. Sarnoski, Sean F. O'Keefe, Michael L. Jahncke, Parameswarakumar Mallikarjunan, George J. Flick

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Traditionally crab meat spoilage has been evaluated using sensory panels. A method was developed using solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) to examine the aroma profile of blue crab (Callinectes sapidus) for chemical indicators of spoilage. The chemicals found to correlate best with spoilage were trimethylamine (TMA), ammonia, and indole over a period of 7 days. In addition, chemicals previously not identified in the aroma profile of blue crab were tentatively detected. Scan mode of the mass spectrometer was used to qualitatively determine compounds extracted from the volatile profile of spoiling blue crab by the SPME fiber. Selected ion monitoring (SIM) mode of the mass spectrometer improved resolution, identified compounds at low concentrations, and allowed spoilage related compounds to be detected in one chromatographic run without sample heating. TMA increased linearly. A significant difference in TMA concentrations were found for day 0 and day 4 samples. Indole concentrations corresponded well with sensory and microbial evaluations, in early, mid, and highly spoiled crab meat samples.

Original languageEnglish (US)
Pages (from-to)930-935
Number of pages6
JournalFood Chemistry
Volume122
Issue number3
DOIs
StatePublished - Oct 1 2010

Fingerprint

crab meat
Brachyura
Spoilage
Callinectes sapidus
Meats
spoilage
Gas chromatography
Gas Chromatography-Mass Spectrometry
Meat
trimethylamine
Mass spectrometry
crabs
meat
Mass spectrometers
indoles
spectrometers
odors
Solid Phase Microextraction
Ammonia
sampling

Keywords

  • Blue crab
  • Gas chromatography
  • Indole
  • Mass spectrometry
  • Solid-phase microextraction
  • Spoilage
  • Trimethylamine

Cite this

Analysis of crab meat volatiles as possible spoilage indicators for blue crab (Callinectes sapidus) meat by gas chromatography-mass spectrometry. / Sarnoski, Paul J.; O'Keefe, Sean F.; Jahncke, Michael L.; Mallikarjunan, Parameswarakumar; Flick, George J.

In: Food Chemistry, Vol. 122, No. 3, 01.10.2010, p. 930-935.

Research output: Contribution to journalArticle

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