Analysis of binding modes of biopanning heptapeptides with specific affinity to chitosan wrapped MSN

Yibing Wang; Yong Yu; Wenji Yao; Min Li; Ping Wang

doi:10.1016/j.bbrc.2020.12.020

Analysis of binding modes of biopanning heptapeptides with specific affinity to chitosan wrapped MSN

Yibing Wang, Yong Yu, Wenji Yao, Min Li, Ping Wang

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

In this work, we demonstrated for the first time that six short heptapeptides screened out by biopanning strategy show affinity binding to chitosan wrapped mesoporous silica nanoparticle (CS/MSN). The interaction between peptide and chitosan-wrapped MSN (CS/MSN) was carefully analyzed with the aid of DLS and ELISA characterization. The results show that π-π stacking and hydrogen bonds account for the strong adsorption under neutral as well as acidic conditions. While hydrophilic and electrostatic interaction are the major modes of the peptide under alkaline condition. These different interaction modes involved in the adsorption of peptides onto CS/MSN could vividly mimic the binding of peptide with chitosan in physiological conditions and help to deeply understand the process of protein adsorption on nanomaterials.

Original language	English (US)
Pages (from-to)	64-69
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	539
DOIs	https://doi.org/10.1016/j.bbrc.2020.12.020
State	Published - Feb 5 2021

Bibliographical note

Funding Information:
This work was supported by the Natural Science Foundation of Shanghai ( 19ZR1412400 ), the National Natural Science Foundation of China ( 21303050 , 31671012 ), and the Fundamental Research Funds for the Central Universities ( 222201514327 ).

Publisher Copyright:
© 2020 Elsevier Inc.

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title = "Analysis of binding modes of biopanning heptapeptides with specific affinity to chitosan wrapped MSN",

abstract = "In this work, we demonstrated for the first time that six short heptapeptides screened out by biopanning strategy show affinity binding to chitosan wrapped mesoporous silica nanoparticle (CS/MSN). The interaction between peptide and chitosan-wrapped MSN (CS/MSN) was carefully analyzed with the aid of DLS and ELISA characterization. The results show that π-π stacking and hydrogen bonds account for the strong adsorption under neutral as well as acidic conditions. While hydrophilic and electrostatic interaction are the major modes of the peptide under alkaline condition. These different interaction modes involved in the adsorption of peptides onto CS/MSN could vividly mimic the binding of peptide with chitosan in physiological conditions and help to deeply understand the process of protein adsorption on nanomaterials.",

author = "Yibing Wang and Yong Yu and Wenji Yao and Min Li and Ping Wang",

note = "Funding Information: This work was supported by the Natural Science Foundation of Shanghai ( 19ZR1412400 ), the National Natural Science Foundation of China ( 21303050 , 31671012 ), and the Fundamental Research Funds for the Central Universities ( 222201514327 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

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doi = "10.1016/j.bbrc.2020.12.020",

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T1 - Analysis of binding modes of biopanning heptapeptides with specific affinity to chitosan wrapped MSN

AU - Wang, Yibing

AU - Yu, Yong

AU - Yao, Wenji

AU - Li, Min

AU - Wang, Ping

N1 - Funding Information: This work was supported by the Natural Science Foundation of Shanghai ( 19ZR1412400 ), the National Natural Science Foundation of China ( 21303050 , 31671012 ), and the Fundamental Research Funds for the Central Universities ( 222201514327 ). Publisher Copyright: © 2020 Elsevier Inc.

PY - 2021/2/5

Y1 - 2021/2/5

N2 - In this work, we demonstrated for the first time that six short heptapeptides screened out by biopanning strategy show affinity binding to chitosan wrapped mesoporous silica nanoparticle (CS/MSN). The interaction between peptide and chitosan-wrapped MSN (CS/MSN) was carefully analyzed with the aid of DLS and ELISA characterization. The results show that π-π stacking and hydrogen bonds account for the strong adsorption under neutral as well as acidic conditions. While hydrophilic and electrostatic interaction are the major modes of the peptide under alkaline condition. These different interaction modes involved in the adsorption of peptides onto CS/MSN could vividly mimic the binding of peptide with chitosan in physiological conditions and help to deeply understand the process of protein adsorption on nanomaterials.

AB - In this work, we demonstrated for the first time that six short heptapeptides screened out by biopanning strategy show affinity binding to chitosan wrapped mesoporous silica nanoparticle (CS/MSN). The interaction between peptide and chitosan-wrapped MSN (CS/MSN) was carefully analyzed with the aid of DLS and ELISA characterization. The results show that π-π stacking and hydrogen bonds account for the strong adsorption under neutral as well as acidic conditions. While hydrophilic and electrostatic interaction are the major modes of the peptide under alkaline condition. These different interaction modes involved in the adsorption of peptides onto CS/MSN could vividly mimic the binding of peptide with chitosan in physiological conditions and help to deeply understand the process of protein adsorption on nanomaterials.

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SP - 64

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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Analysis of binding modes of biopanning heptapeptides with specific affinity to chitosan wrapped MSN

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