Analysis of base excision and nucleotide excision repair in Candida albicans

Melanie Legrand, Christine L. Chan, Peter A. Jauert, David T. Kirkpatrick

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Candida albicans, clinically the most important human fungal pathogen, rapidly develops resistance to antifungal drugs. The acquisition of resistance has been linked to various types of genome changes. As part of an ongoing study of this problem, we investigated mutation, genome stability and drug resistance acquisition in C. albicans strains with deletions in the base excision repair (BER) genes NTG1, APN1 and OGG1, and in the nucleotide excision repair (NER) genes RAD2 and RAD10. The BER mutants did not exhibit any change in their susceptibility to DNA-damaging agents, but the NER mutants were extremely sensitive to UV-induced DNA damage. We did not observe any significant change in mutation, genome stability and antifungal drug sensitivity in the mutant strains we tested. However, we detected a number of intriguing phenotypic differences between strains bearing deletions in equivalent C. albicans and Saccharomyces cerevisiae BER and NER genes, which may be related to differences in the life cycles of these two fungi.

Original languageEnglish (US)
Pages (from-to)2446-2456
Number of pages11
JournalMicrobiology
Volume154
Issue number8
DOIs
StatePublished - Sep 11 2008

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Candida albicans
DNA Repair
Genomic Instability
Fungal Drug Resistance
Genes
Mutation
Life Cycle Stages
Drug Resistance
DNA Damage
Saccharomyces cerevisiae
Fungi
Genome
DNA
Pharmaceutical Preparations

Cite this

Analysis of base excision and nucleotide excision repair in Candida albicans. / Legrand, Melanie; Chan, Christine L.; Jauert, Peter A.; Kirkpatrick, David T.

In: Microbiology, Vol. 154, No. 8, 11.09.2008, p. 2446-2456.

Research output: Contribution to journalArticle

Legrand, Melanie ; Chan, Christine L. ; Jauert, Peter A. ; Kirkpatrick, David T. / Analysis of base excision and nucleotide excision repair in Candida albicans. In: Microbiology. 2008 ; Vol. 154, No. 8. pp. 2446-2456.
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