TY - JOUR
T1 - Analyses of conformational states of the transporter associated with antigen processing (TAP) protein in a native cellular membrane environment
AU - Geng, Jie
AU - Sivaramakrishnan, Sivaraj
AU - Raghavan, Malini
PY - 2013/12/27
Y1 - 2013/12/27
N2 - Background: TAP proteins tagged with CFP and YFP were used for FRET spectroscopy measurements. Results: Conformational changes of the nucleotide binding domains (NBD) were measurable in permeabilized cells. Conclusion: TAP-specific peptides induce NBD closure, and maximal NBD closure is induced by the combination of a peptide and a non-hydrolysable ATP analog. Significance: These studies elucidate distinct steps of the TAP transport cycle.
AB - Background: TAP proteins tagged with CFP and YFP were used for FRET spectroscopy measurements. Results: Conformational changes of the nucleotide binding domains (NBD) were measurable in permeabilized cells. Conclusion: TAP-specific peptides induce NBD closure, and maximal NBD closure is induced by the combination of a peptide and a non-hydrolysable ATP analog. Significance: These studies elucidate distinct steps of the TAP transport cycle.
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U2 - 10.1074/jbc.M113.504696
DO - 10.1074/jbc.M113.504696
M3 - Article
AN - SCOPUS:84891393628
SN - 0021-9258
VL - 288
SP - 37039
EP - 37047
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 52
ER -
