Analyses of conformational states of the transporter associated with antigen processing (TAP) protein in a native cellular membrane environment

Jie Geng, Sivaraj Sivaramakrishnan, Malini Raghavan

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Background: TAP proteins tagged with CFP and YFP were used for FRET spectroscopy measurements. Results: Conformational changes of the nucleotide binding domains (NBD) were measurable in permeabilized cells. Conclusion: TAP-specific peptides induce NBD closure, and maximal NBD closure is induced by the combination of a peptide and a non-hydrolysable ATP analog. Significance: These studies elucidate distinct steps of the TAP transport cycle.

Original languageEnglish (US)
Pages (from-to)37039-37047
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number52
DOIs
StatePublished - Dec 27 2013

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