Analyses of alternative polyadenylation: From old school biochemistry to high-throughput technologies

Hsin Sung Yeh, Wei Zhang, Jeongsik Yong

Research output: Contribution to journalShort survey

3 Scopus citations

Abstract

Alternations in usage of polyadenylation sites during transcription termination yield transcript isoforms from a gene. Recent findings of transcriptome-wide alternative polyadenylation (APA) as a molecular response to changes in biology position APA not only as a molecular event of early transcriptional termination but also as a cellular regulatory step affecting various biological pathways. With the development of high-throughput profiling technologies at a single nucleotide level and their applications targeted to the 3'-end of mRNAs, dynamics in the landscape of mRNA 3'-end is measureable at a global scale. In this review, methods and technologies that have been adopted to study APA events are discussed. In addition, various bioinformatics algorithms for APA isoform analysis using publicly available RNA-seq datasets are introduced.

Original languageEnglish (US)
Pages (from-to)201-207
Number of pages7
JournalBMB Reports
Volume50
Issue number4
DOIs
StatePublished - 2017

Keywords

  • 3' end sequencing methods
  • Alternative polyadenylation
  • Bioinformatics
  • High-throughput sequencing
  • Hybrid sequencing

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