Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.

Liang Wang; Yecong Li; Paul L Chen; Min M Addy; Yifeng Chen; Jun Zhu; R. R Ruan

doi:10.1016/j.biortech.2009.10.062

Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.

Liang Wang, Yecong Li, Paul L Chen, Min M Addy, Yifeng Chen, Jun Zhu, R. R Ruan

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

606 Scopus citations

Abstract

The present study was to investigate the effectiveness of using digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Different dilution multiples of 10, 15, 20, and 25 were applied to the digested manure and algal growth was compared in regard to growth rate, nutrient removal efficiency, and final algal fatty acids content and composition. Slower growth rates were observed with less diluted manure samples with higher turbidities in the initial cultivation days. A reverse linear relationship (R² = 0.982) was found between the average specific growth rate of the beginning 7 days and the initial turbidities. Algae removed ammonia, total nitrogen, total phosphorus, and COD by 100%, 75.7-82.5%, 62.5-74.7%, and 27.4-38.4%, respectively, in differently diluted dairy manure. COD in digested dairy manure, beside CO₂, proved to be another carbon source for mixotrophic Chlorella. Fatty acid profiles derived from triacylglyceride (TAG), phospholipid and free fatty acids showed that octadecadienoic acid (C18:2) and hexadecanoic acid (C16:0) were the two most abundant fatty acids in the algae. The total fatty acid content of the dry weight increased from 9.00% to 13.7% along with the increasing dilution multiples. Based on the results from this study, a process combining anaerobic digestion and algae cultivation can be proposed as an effective way to convert high strength dairy manure into profitable byproducts as well as to reduce contaminations to environment.

Original language	English (US)
Pages (from-to)	2623-2628
Number of pages	6
Journal	Bioresource Technology
Volume	101
Issue number	8
DOIs	https://doi.org/10.1016/j.biortech.2009.10.062
State	Published - Apr 2010

Bibliographical note

Funding Information:
The authors are grateful to Richard Huelskamp for supplying manure samples and Blanca C. Martinez for providing help in the labs. The study was supported in part by grants from the University of Minnesota Initiative for Renewable Energy and the Environment (IREE) and the Center for Biorefining.

Keywords

Algae
Anaerobic digested dairy manure
Chlorella
Lipid content
Nutrients removal

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10.1016/j.biortech.2009.10.062

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title = "Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.",

abstract = "The present study was to investigate the effectiveness of using digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Different dilution multiples of 10, 15, 20, and 25 were applied to the digested manure and algal growth was compared in regard to growth rate, nutrient removal efficiency, and final algal fatty acids content and composition. Slower growth rates were observed with less diluted manure samples with higher turbidities in the initial cultivation days. A reverse linear relationship (R2 = 0.982) was found between the average specific growth rate of the beginning 7 days and the initial turbidities. Algae removed ammonia, total nitrogen, total phosphorus, and COD by 100%, 75.7-82.5%, 62.5-74.7%, and 27.4-38.4%, respectively, in differently diluted dairy manure. COD in digested dairy manure, beside CO2, proved to be another carbon source for mixotrophic Chlorella. Fatty acid profiles derived from triacylglyceride (TAG), phospholipid and free fatty acids showed that octadecadienoic acid (C18:2) and hexadecanoic acid (C16:0) were the two most abundant fatty acids in the algae. The total fatty acid content of the dry weight increased from 9.00% to 13.7% along with the increasing dilution multiples. Based on the results from this study, a process combining anaerobic digestion and algae cultivation can be proposed as an effective way to convert high strength dairy manure into profitable byproducts as well as to reduce contaminations to environment.",

keywords = "Algae, Anaerobic digested dairy manure, Chlorella, Lipid content, Nutrients removal",

author = "Liang Wang and Yecong Li and Chen, {Paul L} and Addy, {Min M} and Yifeng Chen and Jun Zhu and Ruan, {R. R}",

note = "Funding Information: The authors are grateful to Richard Huelskamp for supplying manure samples and Blanca C. Martinez for providing help in the labs. The study was supported in part by grants from the University of Minnesota Initiative for Renewable Energy and the Environment (IREE) and the Center for Biorefining. ",

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AU - Addy, Min M

AU - Chen, Yifeng

AU - Zhu, Jun

AU - Ruan, R. R

N1 - Funding Information: The authors are grateful to Richard Huelskamp for supplying manure samples and Blanca C. Martinez for providing help in the labs. The study was supported in part by grants from the University of Minnesota Initiative for Renewable Energy and the Environment (IREE) and the Center for Biorefining.

PY - 2010/4

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N2 - The present study was to investigate the effectiveness of using digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Different dilution multiples of 10, 15, 20, and 25 were applied to the digested manure and algal growth was compared in regard to growth rate, nutrient removal efficiency, and final algal fatty acids content and composition. Slower growth rates were observed with less diluted manure samples with higher turbidities in the initial cultivation days. A reverse linear relationship (R2 = 0.982) was found between the average specific growth rate of the beginning 7 days and the initial turbidities. Algae removed ammonia, total nitrogen, total phosphorus, and COD by 100%, 75.7-82.5%, 62.5-74.7%, and 27.4-38.4%, respectively, in differently diluted dairy manure. COD in digested dairy manure, beside CO2, proved to be another carbon source for mixotrophic Chlorella. Fatty acid profiles derived from triacylglyceride (TAG), phospholipid and free fatty acids showed that octadecadienoic acid (C18:2) and hexadecanoic acid (C16:0) were the two most abundant fatty acids in the algae. The total fatty acid content of the dry weight increased from 9.00% to 13.7% along with the increasing dilution multiples. Based on the results from this study, a process combining anaerobic digestion and algae cultivation can be proposed as an effective way to convert high strength dairy manure into profitable byproducts as well as to reduce contaminations to environment.

AB - The present study was to investigate the effectiveness of using digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Different dilution multiples of 10, 15, 20, and 25 were applied to the digested manure and algal growth was compared in regard to growth rate, nutrient removal efficiency, and final algal fatty acids content and composition. Slower growth rates were observed with less diluted manure samples with higher turbidities in the initial cultivation days. A reverse linear relationship (R2 = 0.982) was found between the average specific growth rate of the beginning 7 days and the initial turbidities. Algae removed ammonia, total nitrogen, total phosphorus, and COD by 100%, 75.7-82.5%, 62.5-74.7%, and 27.4-38.4%, respectively, in differently diluted dairy manure. COD in digested dairy manure, beside CO2, proved to be another carbon source for mixotrophic Chlorella. Fatty acid profiles derived from triacylglyceride (TAG), phospholipid and free fatty acids showed that octadecadienoic acid (C18:2) and hexadecanoic acid (C16:0) were the two most abundant fatty acids in the algae. The total fatty acid content of the dry weight increased from 9.00% to 13.7% along with the increasing dilution multiples. Based on the results from this study, a process combining anaerobic digestion and algae cultivation can be proposed as an effective way to convert high strength dairy manure into profitable byproducts as well as to reduce contaminations to environment.

KW - Algae

KW - Anaerobic digested dairy manure

KW - Chlorella

KW - Lipid content

KW - Nutrients removal

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ER -

Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.

Abstract

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Keywords

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