An unsought and expensive way to make gold nanoparticles on the way to the development of SiO2@ZrO2 nanocarriers for cancer vaccination

Livia Naszályi Nagy; Evert Dhaene; Imola Csilla Szigyártó; Judith Mihály; Zoltán May; Zoltán Varga; Isabel Van Driessche; José C. Martins; Krisztina Fehér

doi:10.1016/j.molliq.2020.113307

An unsought and expensive way to make gold nanoparticles on the way to the development of SiO₂@ZrO₂ nanocarriers for cancer vaccination

Livia Naszályi Nagy, Evert Dhaene, Imola Csilla Szigyártó, Judith Mihály, Zoltán May, Zoltán Varga, Isabel Van Driessche, José C. Martins, Krisztina Fehér

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Silica@zirconia core@shell oxide nanoparticles of approx. 50 nm diameter were developed in view of an application as a novel carrier with adjuvant activities for cancer vaccines. To this end, the aim was to cover the surface of the nanocarrier by potent single stranded DNA (ssDNA) type immune stimulators used in cancer vaccination. Prior to characterizing the binding of ssDNA to the nanocarrier surface, the adsorption of deoxynucleoside monophosphate building blocks (dNMPs) was studied. After optimization of conditions for synthesis, solvent exchange and surface modification, the effects and possible interference of several buffers, such as HEPES, PIPES, MOPS and MOPSO, were also investigated. Formation of a new compound was revealed by UV–VIS spectroscopy during the recording of zeta potential vs. pH titration curves in the presence of HEPES and PIPES buffer in the neutral-acidic pH region. We identified this new species as gold ions etched from the electrode of the zeta cuvette, stabilized by the buffers via chelation, which is then followed by their conversion into gold nanoparticles.

Original language	English (US)
Article number	113307
Journal	Journal of Molecular Liquids
Volume	311
DOIs	https://doi.org/10.1016/j.molliq.2020.113307
State	Published - Aug 1 2020
Externally published	Yes

Bibliographical note

Funding Information:
This project has received funding from the European Union 's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703374 , FWO Vlaanderen , and Ghent University . The 700 MHz equipment used in this study was funded by the FFEU-ZWAP initiative of the Flemish Government and a Hercules grant ( AUGE15/011 ). K.F. acknowledges the support of the Marie Curie Career Integration Grant ( 303917 PGN-INNATE ) and the Research Grant from the Research Foundation Flanders ( 1508414N and 1525517N ). János Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/004333/18/7) and the New National Excellence Program of Debrecen University (ÚNKP-19-4 Bolyai+). This research was supported by the National Research, Development and Innovation Office of Hungary (grant NKFI/OTKA NN 128368 ).

Funding Information:
This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703374, FWO Vlaanderen, and Ghent University. The 700 MHz equipment used in this study was funded by the FFEU-ZWAP initiative of the Flemish Government and a Hercules grant (AUGE15/011). K.F. acknowledges the support of the Marie Curie Career Integration Grant (303917 PGN-INNATE) and the Research Grant from the Research Foundation Flanders (1508414N and 1525517N). J?nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/004333/18/7) and the New National Excellence Program of Debrecen University (?NKP-19-4 Bolyai+). This research was supported by the National Research, Development and Innovation Office of Hungary (grant NKFI/OTKA NN 128368).

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

Gold
HEPES
Nanoparticle
PIPES
Silica@zirconia
Zeta cuvette electrode

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1016/j.molliq.2020.113307

Find It

Find It at U of M Libraries

Cite this

@article{0332e95e11e44e62ade6b1cea0e87155,

title = "An unsought and expensive way to make gold nanoparticles on the way to the development of SiO2@ZrO2 nanocarriers for cancer vaccination",

abstract = "Silica@zirconia core@shell oxide nanoparticles of approx. 50 nm diameter were developed in view of an application as a novel carrier with adjuvant activities for cancer vaccines. To this end, the aim was to cover the surface of the nanocarrier by potent single stranded DNA (ssDNA) type immune stimulators used in cancer vaccination. Prior to characterizing the binding of ssDNA to the nanocarrier surface, the adsorption of deoxynucleoside monophosphate building blocks (dNMPs) was studied. After optimization of conditions for synthesis, solvent exchange and surface modification, the effects and possible interference of several buffers, such as HEPES, PIPES, MOPS and MOPSO, were also investigated. Formation of a new compound was revealed by UV–VIS spectroscopy during the recording of zeta potential vs. pH titration curves in the presence of HEPES and PIPES buffer in the neutral-acidic pH region. We identified this new species as gold ions etched from the electrode of the zeta cuvette, stabilized by the buffers via chelation, which is then followed by their conversion into gold nanoparticles.",

keywords = "Gold, HEPES, Nanoparticle, PIPES, Silica@zirconia, Zeta cuvette electrode",

author = "Nagy, {Livia Nasz{\'a}lyi} and Evert Dhaene and Szigy{\'a}rt{\'o}, {Imola Csilla} and Judith Mih{\'a}ly and Zolt{\'a}n May and Zolt{\'a}n Varga and {Van Driessche}, Isabel and Martins, {Jos{\'e} C.} and Krisztina Feh{\'e}r",

note = "Funding Information: This project has received funding from the European Union 's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703374 , FWO Vlaanderen , and Ghent University . The 700 MHz equipment used in this study was funded by the FFEU-ZWAP initiative of the Flemish Government and a Hercules grant ( AUGE15/011 ). K.F. acknowledges the support of the Marie Curie Career Integration Grant ( 303917 PGN-INNATE ) and the Research Grant from the Research Foundation Flanders ( 1508414N and 1525517N ). J{\'a}nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/004333/18/7) and the New National Excellence Program of Debrecen University ({\'U}NKP-19-4 Bolyai+). This research was supported by the National Research, Development and Innovation Office of Hungary (grant NKFI/OTKA NN 128368 ). Funding Information: This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703374, FWO Vlaanderen, and Ghent University. The 700 MHz equipment used in this study was funded by the FFEU-ZWAP initiative of the Flemish Government and a Hercules grant (AUGE15/011). K.F. acknowledges the support of the Marie Curie Career Integration Grant (303917 PGN-INNATE) and the Research Grant from the Research Foundation Flanders (1508414N and 1525517N). J?nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/004333/18/7) and the New National Excellence Program of Debrecen University (?NKP-19-4 Bolyai+). This research was supported by the National Research, Development and Innovation Office of Hungary (grant NKFI/OTKA NN 128368). Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2020",

month = aug,

day = "1",

doi = "10.1016/j.molliq.2020.113307",

language = "English (US)",

volume = "311",

journal = "Journal of Molecular Liquids",

issn = "0167-7322",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - An unsought and expensive way to make gold nanoparticles on the way to the development of SiO2@ZrO2 nanocarriers for cancer vaccination

AU - Nagy, Livia Naszályi

AU - Dhaene, Evert

AU - Szigyártó, Imola Csilla

AU - Mihály, Judith

AU - May, Zoltán

AU - Varga, Zoltán

AU - Van Driessche, Isabel

AU - Martins, José C.

AU - Fehér, Krisztina

N1 - Funding Information: This project has received funding from the European Union 's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703374 , FWO Vlaanderen , and Ghent University . The 700 MHz equipment used in this study was funded by the FFEU-ZWAP initiative of the Flemish Government and a Hercules grant ( AUGE15/011 ). K.F. acknowledges the support of the Marie Curie Career Integration Grant ( 303917 PGN-INNATE ) and the Research Grant from the Research Foundation Flanders ( 1508414N and 1525517N ). János Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/004333/18/7) and the New National Excellence Program of Debrecen University (ÚNKP-19-4 Bolyai+). This research was supported by the National Research, Development and Innovation Office of Hungary (grant NKFI/OTKA NN 128368 ). Funding Information: This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 703374, FWO Vlaanderen, and Ghent University. The 700 MHz equipment used in this study was funded by the FFEU-ZWAP initiative of the Flemish Government and a Hercules grant (AUGE15/011). K.F. acknowledges the support of the Marie Curie Career Integration Grant (303917 PGN-INNATE) and the Research Grant from the Research Foundation Flanders (1508414N and 1525517N). J?nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (BO/004333/18/7) and the New National Excellence Program of Debrecen University (?NKP-19-4 Bolyai+). This research was supported by the National Research, Development and Innovation Office of Hungary (grant NKFI/OTKA NN 128368). Publisher Copyright: © 2018 Elsevier B.V.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - Silica@zirconia core@shell oxide nanoparticles of approx. 50 nm diameter were developed in view of an application as a novel carrier with adjuvant activities for cancer vaccines. To this end, the aim was to cover the surface of the nanocarrier by potent single stranded DNA (ssDNA) type immune stimulators used in cancer vaccination. Prior to characterizing the binding of ssDNA to the nanocarrier surface, the adsorption of deoxynucleoside monophosphate building blocks (dNMPs) was studied. After optimization of conditions for synthesis, solvent exchange and surface modification, the effects and possible interference of several buffers, such as HEPES, PIPES, MOPS and MOPSO, were also investigated. Formation of a new compound was revealed by UV–VIS spectroscopy during the recording of zeta potential vs. pH titration curves in the presence of HEPES and PIPES buffer in the neutral-acidic pH region. We identified this new species as gold ions etched from the electrode of the zeta cuvette, stabilized by the buffers via chelation, which is then followed by their conversion into gold nanoparticles.

AB - Silica@zirconia core@shell oxide nanoparticles of approx. 50 nm diameter were developed in view of an application as a novel carrier with adjuvant activities for cancer vaccines. To this end, the aim was to cover the surface of the nanocarrier by potent single stranded DNA (ssDNA) type immune stimulators used in cancer vaccination. Prior to characterizing the binding of ssDNA to the nanocarrier surface, the adsorption of deoxynucleoside monophosphate building blocks (dNMPs) was studied. After optimization of conditions for synthesis, solvent exchange and surface modification, the effects and possible interference of several buffers, such as HEPES, PIPES, MOPS and MOPSO, were also investigated. Formation of a new compound was revealed by UV–VIS spectroscopy during the recording of zeta potential vs. pH titration curves in the presence of HEPES and PIPES buffer in the neutral-acidic pH region. We identified this new species as gold ions etched from the electrode of the zeta cuvette, stabilized by the buffers via chelation, which is then followed by their conversion into gold nanoparticles.

KW - Gold

KW - HEPES

KW - Nanoparticle

KW - PIPES

KW - Silica@zirconia

KW - Zeta cuvette electrode

UR - http://www.scopus.com/inward/record.url?scp=85085206814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85085206814&partnerID=8YFLogxK

U2 - 10.1016/j.molliq.2020.113307

DO - 10.1016/j.molliq.2020.113307

M3 - Article

AN - SCOPUS:85085206814

SN - 0167-7322

VL - 311

JO - Journal of Molecular Liquids

JF - Journal of Molecular Liquids

M1 - 113307

ER -