An unsought and expensive way to make gold nanoparticles on the way to the development of SiO2@ZrO2 nanocarriers for cancer vaccination

Livia Naszályi Nagy, Evert Dhaene, Imola Csilla Szigyártó, Judith Mihály, Zoltán May, Zoltán Varga, Isabel Van Driessche, José C. Martins, Krisztina Fehér

Research output: Contribution to journalArticle

Abstract

Silica@zirconia core@shell oxide nanoparticles of approx. 50 nm diameter were developed in view of an application as a novel carrier with adjuvant activities for cancer vaccines. To this end, the aim was to cover the surface of the nanocarrier by potent single stranded DNA (ssDNA) type immune stimulators used in cancer vaccination. Prior to characterizing the binding of ssDNA to the nanocarrier surface, the adsorption of deoxynucleoside monophosphate building blocks (dNMPs) was studied. After optimization of conditions for synthesis, solvent exchange and surface modification, the effects and possible interference of several buffers, such as HEPES, PIPES, MOPS and MOPSO, were also investigated. Formation of a new compound was revealed by UV–VIS spectroscopy during the recording of zeta potential vs. pH titration curves in the presence of HEPES and PIPES buffer in the neutral-acidic pH region. We identified this new species as gold ions etched from the electrode of the zeta cuvette, stabilized by the buffers via chelation, which is then followed by their conversion into gold nanoparticles.

Original languageEnglish (US)
Article number113307
JournalJournal of Molecular Liquids
Volume311
DOIs
StatePublished - Aug 1 2020
Externally publishedYes

Keywords

  • Gold
  • HEPES
  • Nanoparticle
  • PIPES
  • Silica@zirconia
  • Zeta cuvette electrode

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    Nagy, L. N., Dhaene, E., Szigyártó, I. C., Mihály, J., May, Z., Varga, Z., Van Driessche, I., Martins, J. C., & Fehér, K. (2020). An unsought and expensive way to make gold nanoparticles on the way to the development of SiO2@ZrO2 nanocarriers for cancer vaccination. Journal of Molecular Liquids, 311, [113307]. https://doi.org/10.1016/j.molliq.2020.113307