Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings. We developed a sophisticated, open-source HPLC simulator that is available for free as well as a version for Android users called "HPLC Simulator." Here we discuss a few questions that the simulator can help answer: Why are peaks narrower in gradient elution? What is gradient delay and how does it affect a separation? How much does extracolumn tubing affect a separation?.
|Original language||English (US)|
|Number of pages||8|
|Journal||LC-GC North America|
|State||Published - 2015|
Bibliographical noteFunding Information:
We thank the National Institute of General Medical Sciences of the National Institutes of Health [R01GM098290] for supporting this project. We also thank Jonathan E. Turner, Bonnie A. Alden, and Michael P. Balogh at Waters for helping measure retention data for the Waters stationary phase in the simulator.