An NMR study on the interaction of Escherichia coli Dinl with RecA-ssDNA complexes

Masatoshi Yoshimasu, Hideki Aihara, Yutaka Ito, Sundaresan Rajesh, Satoko Ishibe, Tsutomu Mikawa, Shigeyuki Yokoyama, Takehiko Shibata

Research output: Contribution to journalReview article

21 Citations (Scopus)

Abstract

The SOS response, a set of cellular phenomena exhibited by eubacteria, is initiated by various causes that include DNA damage-induced replication arrest, and is positively regulated by the co-protease activity of RecA. Escherichia coli DinI, a LexA-regulated SOS gene product, shuts off the initiation of the SOS response when overexpressed in vivo. Biochemical and genetic studies indicated that DinI physically interacts with RecA to inhibit its co-protease activity. Using nuclear magnetic resonance (NMR) spectroscopy, we show that DinI tightly binds to the central region of RecA (between the N- and C-terminal domains) and that this interaction is enhanced upon the oligomerisation of RecA. On the other hand, DinI did not inhibit the interaction between 4mer single-stranded (ss)DNA and RecA-ATPγS, but had a slight effect on the structure of ssDNA-RecA-ATPγS complexes involving 8mer and 12mer ssDNA. We hypothesise that prevention of repressor binding to the intermolecular cleft region of RecA protomers by DinI, with the possibility of a slight conformational change induced in the DinI-bound ssDNA-RecA-ATPγS complex, together function to inhibit the co-protease activity of RecA.

Original languageEnglish (US)
Pages (from-to)1735-1743
Number of pages9
JournalNucleic acids research
Volume31
Issue number6
DOIs
StatePublished - Mar 15 2003

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Peptide Hydrolases
Magnetic Resonance Spectroscopy
Escherichia coli
Single-Stranded DNA
Protein Subunits
DNA Damage
Molecular Biology
Bacteria
Genes

Cite this

Yoshimasu, M., Aihara, H., Ito, Y., Rajesh, S., Ishibe, S., Mikawa, T., ... Shibata, T. (2003). An NMR study on the interaction of Escherichia coli Dinl with RecA-ssDNA complexes. Nucleic acids research, 31(6), 1735-1743. https://doi.org/10.1093/nar/gkg256

An NMR study on the interaction of Escherichia coli Dinl with RecA-ssDNA complexes. / Yoshimasu, Masatoshi; Aihara, Hideki; Ito, Yutaka; Rajesh, Sundaresan; Ishibe, Satoko; Mikawa, Tsutomu; Yokoyama, Shigeyuki; Shibata, Takehiko.

In: Nucleic acids research, Vol. 31, No. 6, 15.03.2003, p. 1735-1743.

Research output: Contribution to journalReview article

Yoshimasu, M, Aihara, H, Ito, Y, Rajesh, S, Ishibe, S, Mikawa, T, Yokoyama, S & Shibata, T 2003, 'An NMR study on the interaction of Escherichia coli Dinl with RecA-ssDNA complexes', Nucleic acids research, vol. 31, no. 6, pp. 1735-1743. https://doi.org/10.1093/nar/gkg256
Yoshimasu, Masatoshi ; Aihara, Hideki ; Ito, Yutaka ; Rajesh, Sundaresan ; Ishibe, Satoko ; Mikawa, Tsutomu ; Yokoyama, Shigeyuki ; Shibata, Takehiko. / An NMR study on the interaction of Escherichia coli Dinl with RecA-ssDNA complexes. In: Nucleic acids research. 2003 ; Vol. 31, No. 6. pp. 1735-1743.
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