An isocratic liquid chromatography method for determining HIV non-nucleoside reverse transcriptase inhibitor and protease inhibitor concentrations in human plasma

Dennis R. Weller, Richard C. Brundage, Henry H. Balfour, Heather E. Vezina

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

An efficient, isocratic high performance liquid chromatography (HPLC) method for determining human immunodeficiency virus (HIV) non-nucleoside reverse transcriptase inhibitors (NNRTIs) and protease inhibitors (PIs) in plasma is advantageous for laboratories participating in clinical trials and therapeutic drug monitoring (TDM) programs, or conducting small animal research. The combination of isocratic reversed phase chromatography using an S-3, 3.0 mm × 150 mm column along with low plasma volume (200 μl), rapid liquid-liquid extraction, and detection at a single wavelength (212 nm) over a short run time makes this method valuable. Within and between assay variability ranges from 0.8 to 3.5% and 1.2-6.2%, respectively. Accuracy ranges from 91.0 to 112.8% for four quality controls (50, 100, 1000, and 10,000 ng/ml) for all drugs measured (efavirenz, nevirapine, amprenavir, atazanavir, indinavir, lopinavir, nelfinavir, ritonavir, and saquinavir).

Original languageEnglish (US)
Pages (from-to)369-373
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume848
Issue number2
DOIs
StatePublished - Apr 1 2007

Keywords

  • HIV
  • HPLC-UV
  • Isocratic
  • Liquid-liquid extraction
  • Non-nucleoside reverse transcriptase inhibitors
  • Protease inhibitors
  • Therapeutic drug monitoring

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