An Ion‐Pair High Performance Liquid Chromatographic Method for the Determination of Aspartame and its Decomposition Products

JEFFREY A. STAMP, Ted P Labuza

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The retention behavior of aspartame and six decomposition products were evaluated as a function of added sulfonic acid ion‐pair mobile phase modifiers by HPLC. The separation of aspartame decomposition products was optimized utilizing an isocratic mobile phase of acetonitrile, monosodium phosphate buffer, and I‐heptane sulfonatc at pH 3 with UV detection at 214 nm. The effects of ion‐pair chain length and mobile phase pH on the separation and quantitation of six aspartame decomposition products were compared to traditional reversed‐phase methods. The advantage of the described method is a rapid analysis with minimal peak tailing, which is easily manipulated to maintain the desired resolution as chromatographic conditions change.

Original languageEnglish (US)
Pages (from-to)1043-1046
Number of pages4
JournalJournal of food science
Volume54
Issue number4
DOIs
StatePublished - Jul 1989

Fingerprint Dive into the research topics of 'An Ion‐Pair High Performance Liquid Chromatographic Method for the Determination of Aspartame and its Decomposition Products'. Together they form a unique fingerprint.

Cite this