An induced chromosomal translocation in soybean disrupts a KASI ortholog and is associated with a high-sucrose and low-oil seed phenotype

Austin A. Dobbels, Jean Michel Michno, Benjamin W. Campbell, Kamaldeep S. Virdi, Adrian O. Stec, Gary J. Muehlbauer, Seth L. Naeve, Robert M. Stupar

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Mutagenesis is a useful tool in many crop species to induce heritable genetic variability for trait improvement and gene discovery. In this study, forward screening of a soybean fast neutron (FN) mutant population identified an individual that produced seed with nearly twice the amount of sucrose (8.1% on dry matter basis) and less than half the amount of oil (8.5% on dry matter basis) as compared to wild type. Bulked segregant analysis (BSA), comparative genomic hybridization, and genome resequencing were used to associate the seed composition phenotype with a reciprocal translocation between chromosomes 8 and 13. In a backcross population, the translocation perfectly cosegregated with the seed composition phenotype and exhibited non-Mendelian segregation patterns. We hypothesize that the translocation is responsible for the altered seed composition by disrupting a β-ketoacyl-[acyl carrier protein] synthase 1 (KASI) ortholog. KASI is a core fatty acid synthesis enzyme that is involved in the conversion of sucrose into oil in developing seeds. This finding may lead to new research directions for developing soybean cultivars with modified carbohydrate and oil seed composition.

Original languageEnglish (US)
Pages (from-to)1215-1223
Number of pages9
JournalG3: Genes, Genomes, Genetics
Volume7
Issue number4
DOIs
StatePublished - Apr 1 2017

Bibliographical note

Funding Information:
The authors are grateful to Dimitri von Ruckert, Jill Miller-Garvin, and Jeffrey Roessler for project support, and acknowledge the Minnesota Supercomputing Institute at the University of Minnesota for providing resources that contributed to the research results reported within this paper (URL: http://www.msi.umn.edu). This work was supported by the Minnesota Soybean Research and Promotion Council (#19-16C), the United Soybean Board (#1520-532-5603), and the National Science Foundation (#MCB-1444581). Author contributions: A.A.D., R.M.S., S.L.N., and G.J.M. conceived this study. A.A.D., J.-M.M., B.W.C., K.S.V., and A.O.S. performed the research. A.A.D., S.L.N., and R.M.S. wrote the manuscript.

Keywords

  • Fast neutron
  • Oil
  • Soybean
  • Sucrose
  • Translocation

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