An in vitro inverted vertical invasion assay to avoid manipulation of rare or sensitive cell types

Tanner J. McArdle, Brenda M. Ogle, Felicite K. Noubissi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The ability to quantify cell migration and invasion is critical in the study of cancer metastasis. Current invasion assays, such as the Boyden Chamber, present difficulties in the measurement of the invasion of cells that are few in number and are intrinsically tied to the cell microenvironment. There exists a need for a three-dimensional invasion assay that is easily reproduced, accessible for most laboratories, and requires no displacement of cells from their original microenvironment. Here we present a simple design for an inverted vertical invasion assay able to assess the invasion capabilities of cells in a three dimensional, extracellular matrix-based environment without displacement from the original culture location. We used the assay to determine the migratory capacity of hybrids between mesenchymal/multipotent stem/stroma cells (MSCs) and breast cancer cells MCF7. These hybrids are formed reliably but rarely (1 in 1,000 cells) and for this reason require an invasion assay that does not involve extensive cell manipulation. Using this assay, we found that MSCs, breast cancer cells, and corresponding fusion products are able to migrate and invade through the extracellular matrix and that hybrids invade in a manner more similar to stromal cells than cancer cells. Thus, this assay can aid the study of the invasive capacity of both cancerous cells and associated fusion hybrids and could augment testing of therapeutic strategies to inhibit metastatic spread.

Original languageEnglish (US)
Pages (from-to)2333-2340
Number of pages8
JournalJournal of Cancer
Volume7
Issue number15
DOIs
StatePublished - Jan 1 2016

Fingerprint

Multipotent Stem Cells
Cell Fusion
Extracellular Matrix
Breast Neoplasms
Cellular Microenvironment
MCF-7 Cells
Stromal Cells
Cell Movement
In Vitro Techniques
Neoplasms
Neoplasm Metastasis
Therapeutics

Keywords

  • 3D assay
  • Collagen
  • Hybrids
  • Invasion
  • Metastasis

Cite this

An in vitro inverted vertical invasion assay to avoid manipulation of rare or sensitive cell types. / McArdle, Tanner J.; Ogle, Brenda M.; Noubissi, Felicite K.

In: Journal of Cancer, Vol. 7, No. 15, 01.01.2016, p. 2333-2340.

Research output: Contribution to journalArticle

McArdle, Tanner J. ; Ogle, Brenda M. ; Noubissi, Felicite K. / An in vitro inverted vertical invasion assay to avoid manipulation of rare or sensitive cell types. In: Journal of Cancer. 2016 ; Vol. 7, No. 15. pp. 2333-2340.
@article{5e2ab391c89343148f5c159b23330352,
title = "An in vitro inverted vertical invasion assay to avoid manipulation of rare or sensitive cell types",
abstract = "The ability to quantify cell migration and invasion is critical in the study of cancer metastasis. Current invasion assays, such as the Boyden Chamber, present difficulties in the measurement of the invasion of cells that are few in number and are intrinsically tied to the cell microenvironment. There exists a need for a three-dimensional invasion assay that is easily reproduced, accessible for most laboratories, and requires no displacement of cells from their original microenvironment. Here we present a simple design for an inverted vertical invasion assay able to assess the invasion capabilities of cells in a three dimensional, extracellular matrix-based environment without displacement from the original culture location. We used the assay to determine the migratory capacity of hybrids between mesenchymal/multipotent stem/stroma cells (MSCs) and breast cancer cells MCF7. These hybrids are formed reliably but rarely (1 in 1,000 cells) and for this reason require an invasion assay that does not involve extensive cell manipulation. Using this assay, we found that MSCs, breast cancer cells, and corresponding fusion products are able to migrate and invade through the extracellular matrix and that hybrids invade in a manner more similar to stromal cells than cancer cells. Thus, this assay can aid the study of the invasive capacity of both cancerous cells and associated fusion hybrids and could augment testing of therapeutic strategies to inhibit metastatic spread.",
keywords = "3D assay, Collagen, Hybrids, Invasion, Metastasis",
author = "McArdle, {Tanner J.} and Ogle, {Brenda M.} and Noubissi, {Felicite K.}",
year = "2016",
month = "1",
day = "1",
doi = "10.7150/jca.15812",
language = "English (US)",
volume = "7",
pages = "2333--2340",
journal = "Journal of Cancer",
issn = "1837-9664",
publisher = "Ivyspring International Publisher",
number = "15",

}

TY - JOUR

T1 - An in vitro inverted vertical invasion assay to avoid manipulation of rare or sensitive cell types

AU - McArdle, Tanner J.

AU - Ogle, Brenda M.

AU - Noubissi, Felicite K.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The ability to quantify cell migration and invasion is critical in the study of cancer metastasis. Current invasion assays, such as the Boyden Chamber, present difficulties in the measurement of the invasion of cells that are few in number and are intrinsically tied to the cell microenvironment. There exists a need for a three-dimensional invasion assay that is easily reproduced, accessible for most laboratories, and requires no displacement of cells from their original microenvironment. Here we present a simple design for an inverted vertical invasion assay able to assess the invasion capabilities of cells in a three dimensional, extracellular matrix-based environment without displacement from the original culture location. We used the assay to determine the migratory capacity of hybrids between mesenchymal/multipotent stem/stroma cells (MSCs) and breast cancer cells MCF7. These hybrids are formed reliably but rarely (1 in 1,000 cells) and for this reason require an invasion assay that does not involve extensive cell manipulation. Using this assay, we found that MSCs, breast cancer cells, and corresponding fusion products are able to migrate and invade through the extracellular matrix and that hybrids invade in a manner more similar to stromal cells than cancer cells. Thus, this assay can aid the study of the invasive capacity of both cancerous cells and associated fusion hybrids and could augment testing of therapeutic strategies to inhibit metastatic spread.

AB - The ability to quantify cell migration and invasion is critical in the study of cancer metastasis. Current invasion assays, such as the Boyden Chamber, present difficulties in the measurement of the invasion of cells that are few in number and are intrinsically tied to the cell microenvironment. There exists a need for a three-dimensional invasion assay that is easily reproduced, accessible for most laboratories, and requires no displacement of cells from their original microenvironment. Here we present a simple design for an inverted vertical invasion assay able to assess the invasion capabilities of cells in a three dimensional, extracellular matrix-based environment without displacement from the original culture location. We used the assay to determine the migratory capacity of hybrids between mesenchymal/multipotent stem/stroma cells (MSCs) and breast cancer cells MCF7. These hybrids are formed reliably but rarely (1 in 1,000 cells) and for this reason require an invasion assay that does not involve extensive cell manipulation. Using this assay, we found that MSCs, breast cancer cells, and corresponding fusion products are able to migrate and invade through the extracellular matrix and that hybrids invade in a manner more similar to stromal cells than cancer cells. Thus, this assay can aid the study of the invasive capacity of both cancerous cells and associated fusion hybrids and could augment testing of therapeutic strategies to inhibit metastatic spread.

KW - 3D assay

KW - Collagen

KW - Hybrids

KW - Invasion

KW - Metastasis

UR - http://www.scopus.com/inward/record.url?scp=85007062113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007062113&partnerID=8YFLogxK

U2 - 10.7150/jca.15812

DO - 10.7150/jca.15812

M3 - Article

C2 - 27994672

AN - SCOPUS:85007062113

VL - 7

SP - 2333

EP - 2340

JO - Journal of Cancer

JF - Journal of Cancer

SN - 1837-9664

IS - 15

ER -