An improved procedure for bile acid extraction and purification and tissue distribution in the rat

Patricia L. Locket, Daniel D. Gallaher

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Two bile acid extraction procedures were compared using endogenously radiolabeled tissues and feces. The method of Setchell et al. (J. Lipid Res. 24, 1085-1100, 1983) resulted in essential complete extraction, whereas that of Manes and Schneider (J. Lipid Res. 6, 376-377, 1971) gave recoveries between 56-82%. The time requirement for the method of Setchell et al. could be drastically reduced with no loss in extraction efficiency. Using extracts from endogenously labeled material, a purification procedure using C18 solid-phase extraction cartridges was developed that recovers >90% of bile acids. The distribution of bile acids within the intestinal tract and liver of the rat was determined.

Original languageEnglish (US)
Pages (from-to)221-223
Number of pages3
JournalLipids
Volume24
Issue number3
DOIs
StatePublished - Mar 1989
Externally publishedYes

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