An improved enzymatic radioassay for ribose 1-phosphate

R. Scott McIvor, Robert M. Wohlhueter, Peter G.W. Plagemann

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A method for measuring ribose 1-phosphate in cell extracts is described. Cell extracts are first fractionated on polyethyleneimine-impregnated cellulose columns to remove nucleoside and base components which otherwise interfere with the enzymatic assay. Ribose 1-phosphate in the eluate is made limiting for the conversion of [14C]hypoxanthine to [14C]inosine in the presence of purine nucleoside phosphorylase. Labeled substrate and product are then easily separated on boronate gel columns or by paper chromatography.

Original languageEnglish (US)
Pages (from-to)150-154
Number of pages5
JournalAnalytical Biochemistry
Volume127
Issue number1
DOIs
StatePublished - Nov 15 1982

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