An improved assay for cyclic GMP using an insect binding protein

Ann Marie Fallon; G. R. Wyatt

doi:10.1016/0003-2697(75)90389-9

An improved assay for cyclic GMP using an insect binding protein

Ann Marie Fallon
, G. R. Wyatt

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Protein from silkmoth pupal fat body was used to develop a simple radioisotope-competition binding assay for cGMP, with increased sensitivity and specificity over the currently used protein binding assay (2). With this assay, we have initiated a study of the distribution of cGMP in crickets, which contain unusually high levels, as previously reported by Ishikawa et al. (11).

Original language	English (US)
Pages (from-to)	614-619
Number of pages	6
Journal	Analytical Biochemistry
Volume	63
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(75)90389-9
State	Published - Feb 1975
Externally published	Yes

Bibliographical note

Funding Information:
’ This work was supported by Grants from the National Research Council of Canada (A6954) and the National Institutes of Health, U.S. Public Health Service (HD-07951).

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10.1016/0003-2697(75)90389-9

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title = "An improved assay for cyclic GMP using an insect binding protein",

abstract = "Protein from silkmoth pupal fat body was used to develop a simple radioisotope-competition binding assay for cGMP, with increased sensitivity and specificity over the currently used protein binding assay (2). With this assay, we have initiated a study of the distribution of cGMP in crickets, which contain unusually high levels, as previously reported by Ishikawa et al. (11).",

author = "Fallon, \{Ann Marie\} and Wyatt, \{G. R.\}",

note = "Funding Information: {\textquoteright} This work was supported by Grants from the National Research Council of Canada (A6954) and the National Institutes of Health, U.S. Public Health Service (HD-07951).",

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language = "English (US)",

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AU - Wyatt, G. R.

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PY - 1975/2

Y1 - 1975/2

N2 - Protein from silkmoth pupal fat body was used to develop a simple radioisotope-competition binding assay for cGMP, with increased sensitivity and specificity over the currently used protein binding assay (2). With this assay, we have initiated a study of the distribution of cGMP in crickets, which contain unusually high levels, as previously reported by Ishikawa et al. (11).

AB - Protein from silkmoth pupal fat body was used to develop a simple radioisotope-competition binding assay for cGMP, with increased sensitivity and specificity over the currently used protein binding assay (2). With this assay, we have initiated a study of the distribution of cGMP in crickets, which contain unusually high levels, as previously reported by Ishikawa et al. (11).

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DO - 10.1016/0003-2697(75)90389-9

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An improved assay for cyclic GMP using an insect binding protein

Abstract

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