Protein from silkmoth pupal fat body was used to develop a simple radioisotope-competition binding assay for cGMP, with increased sensitivity and specificity over the currently used protein binding assay (2). With this assay, we have initiated a study of the distribution of cGMP in crickets, which contain unusually high levels, as previously reported by Ishikawa et al. (11).
Bibliographical noteFunding Information:
’ This work was supported by Grants from the National Research Council of Canada (A6954) and the National Institutes of Health, U.S. Public Health Service (HD-07951).