An HPLC-ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Flavie Bidel, Sophie Corvaisier, Christelle Jozet-Alves, Ivannah Pottier, François Dauphin, Nadège Naud, Cécile Bellanger

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The cuttlefish belongs to the mollusk class Cephalopoda, considered as the most advanced marine invertebrates and thus widely used as models to study the biology of complex behaviors and cognition, as well as their related neurochemical mechanisms. Surprisingly, methods to quantify the biogenic monoamines and their metabolites in cuttlefish brain remain sparse and measure a limited number of analytes. This work aims to validate an HPLC-ECD method for the simultaneous quantification of dopamine, serotonin, norepinephrine and their main metabolites in cuttlefish brain. In comparison and in order to develop a method suitable to answer both ecological and biomedical questions, the validation was also carried out on a phylogenetically remote species: mouse (mammals). The method was shown to be accurate, precise, selective, repeatable and sensitive over a wide range of concentrations for 5-hydroxyindole-3-acetic acid, serotonin, dopamine, 3,4-dihydroxyphenylacetic acid and norepinephrine in the both extracts of cuttlefish and mouse brain, though with low precision and recovery for 4-hydroxy-3-methoxyphenylethylene glycol. Homovanillic acid, accurately studied in rodents, was not detectable in the brain of cuttlefish. Overall, we described here the first fully validated HPLC method for the routine measurement of both monoamines and metabolites in cuttlefish brain.

Original languageEnglish (US)
Pages (from-to)1175-1183
Number of pages9
JournalBiomedical Chromatography
Issue number8
StatePublished - Aug 1 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2016 John Wiley & Sons, Ltd.


  • biogenic monoamines
  • cephalopods
  • dopamine
  • high-performance liquid chromatography–electrochemical detection
  • rodents
  • serotonin


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