An expedient synthesis of fluorescent labeled ceramide-1-phosphate analogues

I. A. Boldyrev, R. E. Brown, J. G. Molotkovsky

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A synthesis for fluorescent analogs of ceramide-1-phosphate bearing 9-anthrylvinyl or 4,4-difluoro-3a,4a-diaza-s-indacene-8-yl (Me 4-BODIPY) fluorophore at co-position of fatty acid residue was carried out. The key stage of the synthesis is hydrolysis of corresponding sphingomyelins catalyzed by phospholipase D from Streptomyces chromofuscus; the enzymatic yield has been raised to 50-70% by appliance of organic solvent in the incubation medium.

Original languageEnglish (US)
Pages (from-to)539-542
Number of pages4
JournalRussian Journal of Bioorganic Chemistry
Volume39
Issue number5
DOIs
StatePublished - Sep 1 2013

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Keywords

  • BODIPY
  • anthrylvinyl
  • ceramide-1-phosphate
  • fluorescent lipid probes
  • phospholipase D
  • synthesis

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