An EXAFS Study of the Interaction of Substrate with the Ferric Active Site of Protocatechuate 3,4-Dioxygenase

Anne E. True, Allen M. Orville, Linda L. Pearce, John D. Lipscomb, Lawrence Que

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


X-ray crystallographic studies of the intradiol cleaving protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa have shown that the enzyme has a trigonal bipyramidal ferric active site with two histidines, two tyrosines, and a solvent molecule as ligands [Ohlendorf, D. H., Lipscomb, J. D., & Weber, P. C. (1988) Nature 336, 403-405]. Fe K-edge EXAFS studies of the spectroscopically similar protocatechuate 3,4-dioxygenase from Brevibacterium fuscum are consistent with a pentacoordinate geometry of the iron active site with 3 O/N ligands at 1.90 Å and 2 O/N ligands at 2.08 Å. The 2.08-Å bonds are assigned to the two histidines, while the 1,90-Å bonds are associated with the two tyrosines and the coordinated solvent. The short Fe-O distance for the solvent suggests that it coordinates as hydroxide rather than water. When the inhibitor terephthalate is bound to the enzyme, the XANES data indicate that the ferric site becomes 6-coordinate and the EXAFS data show a beat pattern which can only be simulated with an additional Fe-O/N interaction at 2.46 Å. Together, the data suggest that the oxygens of the carboxylate group in terephthalate displace the hydroxide and chelate to the ferric site but in an asymmetric fashion. In contrast, protocatechuate 3,4-dioxygenase remains 5-coordinate upon the addition of the slow substrate homoprotocatechuic acid (HPCA). Previous EPR data have indicated that HPCA forms an iron chelate via the two hydroxyl functions. For the iron site to remain 5-coordinate and the HPCA to be chelated to the iron, the substrate must displace not only the hydroxide but also a ligand from the protein backbone, probably a histidine. The mechanistic implications of the displacement of hydroxide and a protein ligand in the active site are discussed.

Original languageEnglish (US)
Pages (from-to)10847-10854
Number of pages8
Issue number48
StatePublished - Dec 1 1990


Dive into the research topics of 'An EXAFS Study of the Interaction of Substrate with the Ferric Active Site of Protocatechuate 3,4-Dioxygenase'. Together they form a unique fingerprint.

Cite this