AN ENDOTHELIALIZED, OXYGEN-TUNABLE MICROFLUIDIC PLATFORM FOR THE STUDY OF SICKLE CELL DISEASE

Samantha R. Schad; Wilbur A. Lam; David K. Wood

AN ENDOTHELIALIZED, OXYGEN-TUNABLE MICROFLUIDIC PLATFORM FOR THE STUDY OF SICKLE CELL DISEASE

Samantha R. Schad, Wilbur A. Lam, David K. Wood

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Sickle cell disease (SCD) is characterized by polymerization of sickle hemoglobin and resultant hemolytic anemia, painful vaso-occlusive crisis (VOC), and multiorgan damage. To better investigate underlying mechanisms of SCD morbidity and mortality, an in vitro microfluidic model of SCD has been developed with both a bloodperfusable endothelialized microvasculature and a means of tuning oxygen to induce red blood cell (RBC) sickling in real-time. The microfluidic model presented here will serve as a comprehensive, physiologically relevant model of SCD to study underlying disease mechanisms and may also offer potential to investigate therapeutic effect of emerging drugs and blood transfusions in future studies.

Original language	English (US)
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	201-202
Number of pages	2
ISBN (Electronic)	9781733419031
State	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: Oct 10 2021 → Oct 14 2021

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 10/14/21

Bibliographical note

Funding Information:
Funding was provided by the NIH under R01 HL140589. Portions of this work were conducted at the Minnesota Nano Center, which is supported by NSF through the NNCI network under ECCS-1542202. We would also like to thank collaborators Yumiko Sakurai and Dr. Melissa Kemp for their valuable input.

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

Endothelialized Microfluidics
Hypoxia
Immunofluorescence
Sickle Cell Disease

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Schad, S. R., Lam, W. A., & Wood, D. K. (2021). AN ENDOTHELIALIZED, OXYGEN-TUNABLE MICROFLUIDIC PLATFORM FOR THE STUDY OF SICKLE CELL DISEASE. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 201-202). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

AN ENDOTHELIALIZED, OXYGEN-TUNABLE MICROFLUIDIC PLATFORM FOR THE STUDY OF SICKLE CELL DISEASE. / Schad, Samantha R.; Lam, Wilbur A.; Wood, David K.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 201-202 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Schad, SR, Lam, WA & Wood, DK 2021, AN ENDOTHELIALIZED, OXYGEN-TUNABLE MICROFLUIDIC PLATFORM FOR THE STUDY OF SICKLE CELL DISEASE. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 201-202, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.

Schad SR, Lam WA, Wood DK. AN ENDOTHELIALIZED, OXYGEN-TUNABLE MICROFLUIDIC PLATFORM FOR THE STUDY OF SICKLE CELL DISEASE. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 201-202. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Schad, Samantha R. ; Lam, Wilbur A. ; Wood, David K. / AN ENDOTHELIALIZED, OXYGEN-TUNABLE MICROFLUIDIC PLATFORM FOR THE STUDY OF SICKLE CELL DISEASE. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 201-202 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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abstract = "Sickle cell disease (SCD) is characterized by polymerization of sickle hemoglobin and resultant hemolytic anemia, painful vaso-occlusive crisis (VOC), and multiorgan damage. To better investigate underlying mechanisms of SCD morbidity and mortality, an in vitro microfluidic model of SCD has been developed with both a bloodperfusable endothelialized microvasculature and a means of tuning oxygen to induce red blood cell (RBC) sickling in real-time. The microfluidic model presented here will serve as a comprehensive, physiologically relevant model of SCD to study underlying disease mechanisms and may also offer potential to investigate therapeutic effect of emerging drugs and blood transfusions in future studies.",

keywords = "Endothelialized Microfluidics, Hypoxia, Immunofluorescence, Sickle Cell Disease",

author = "Schad, {Samantha R.} and Lam, {Wilbur A.} and Wood, {David K.}",

note = "Funding Information: Funding was provided by the NIH under R01 HL140589. Portions of this work were conducted at the Minnesota Nano Center, which is supported by NSF through the NNCI network under ECCS-1542202. We would also like to thank collaborators Yumiko Sakurai and Dr. Melissa Kemp for their valuable input. Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

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AU - Schad, Samantha R.

AU - Lam, Wilbur A.

AU - Wood, David K.

N1 - Funding Information: Funding was provided by the NIH under R01 HL140589. Portions of this work were conducted at the Minnesota Nano Center, which is supported by NSF through the NNCI network under ECCS-1542202. We would also like to thank collaborators Yumiko Sakurai and Dr. Melissa Kemp for their valuable input. Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Sickle cell disease (SCD) is characterized by polymerization of sickle hemoglobin and resultant hemolytic anemia, painful vaso-occlusive crisis (VOC), and multiorgan damage. To better investigate underlying mechanisms of SCD morbidity and mortality, an in vitro microfluidic model of SCD has been developed with both a bloodperfusable endothelialized microvasculature and a means of tuning oxygen to induce red blood cell (RBC) sickling in real-time. The microfluidic model presented here will serve as a comprehensive, physiologically relevant model of SCD to study underlying disease mechanisms and may also offer potential to investigate therapeutic effect of emerging drugs and blood transfusions in future studies.

AB - Sickle cell disease (SCD) is characterized by polymerization of sickle hemoglobin and resultant hemolytic anemia, painful vaso-occlusive crisis (VOC), and multiorgan damage. To better investigate underlying mechanisms of SCD morbidity and mortality, an in vitro microfluidic model of SCD has been developed with both a bloodperfusable endothelialized microvasculature and a means of tuning oxygen to induce red blood cell (RBC) sickling in real-time. The microfluidic model presented here will serve as a comprehensive, physiologically relevant model of SCD to study underlying disease mechanisms and may also offer potential to investigate therapeutic effect of emerging drugs and blood transfusions in future studies.

KW - Endothelialized Microfluidics

KW - Hypoxia

KW - Immunofluorescence

KW - Sickle Cell Disease

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M3 - Conference contribution

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T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 201

EP - 202

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

AN ENDOTHELIALIZED, OXYGEN-TUNABLE MICROFLUIDIC PLATFORM FOR THE STUDY OF SICKLE CELL DISEASE

Abstract

Publication series

Conference

Bibliographical note

Keywords

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