An efficient synthesis of RNA containing GS-441524: The nucleoside precursor of remdesivir

Ramkumar Moorthy; Samantha A Kennelly; Deborah J. Rodriguez; Daniel A. Harki

doi:10.1039/d1ra06589k

An efficient synthesis of RNA containing GS-441524: The nucleoside precursor of remdesivir

Ramkumar Moorthy, Samantha A Kennelly, Deborah J. Rodriguez, Daniel A. Harki

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Remdesivir is an antiviral nucleoside phosphoramidate with activity against multiple viruses, including SARS-CoV-2. To enable studies of viral polymerases with RNA containing remdesivir, we report an efficient synthesis of a phosphoramidite of GS-441524, the nucleoside precursor of remdesivir, and its incorporation into RNA using automated solid-phase RNA synthesis.

Original language	English (US)
Pages (from-to)	31373-31376
Number of pages	4
Journal	RSC Advances
Volume	11
Issue number	50
DOIs	https://doi.org/10.1039/d1ra06589k
State	Published - Sep 14 2021

Bibliographical note

Funding Information:
We gratefully acknowledge the NIH (P01-CA234228) and the University of Minnesota for nancial support. Mass spectrometry was performed at the University of Minnesota Masonic Cancer Center Analytical Biochemistry Core Facility, which is supported by the NIH (P30-CA77598).

Publisher Copyright:
© 2021 The Royal Society of Chemistry.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1039/d1ra06589k

Find It

Find It at U of M Libraries

Cite this

@article{2a7dc03950e446bc80a204ccff65e644,

title = "An efficient synthesis of RNA containing GS-441524: The nucleoside precursor of remdesivir",

abstract = "Remdesivir is an antiviral nucleoside phosphoramidate with activity against multiple viruses, including SARS-CoV-2. To enable studies of viral polymerases with RNA containing remdesivir, we report an efficient synthesis of a phosphoramidite of GS-441524, the nucleoside precursor of remdesivir, and its incorporation into RNA using automated solid-phase RNA synthesis.",

author = "Ramkumar Moorthy and Kennelly, {Samantha A} and Rodriguez, {Deborah J.} and Harki, {Daniel A.}",

note = "Funding Information: We gratefully acknowledge the NIH (P01-CA234228) and the University of Minnesota for nancial support. Mass spectrometry was performed at the University of Minnesota Masonic Cancer Center Analytical Biochemistry Core Facility, which is supported by the NIH (P30-CA77598). Publisher Copyright: {\textcopyright} 2021 The Royal Society of Chemistry.",

year = "2021",

month = sep,

day = "14",

doi = "10.1039/d1ra06589k",

language = "English (US)",

volume = "11",

pages = "31373--31376",

journal = "RSC Advances",

issn = "2046-2069",

publisher = "Royal Society of Chemistry",

number = "50",

}

TY - JOUR

T1 - An efficient synthesis of RNA containing GS-441524

T2 - The nucleoside precursor of remdesivir

AU - Moorthy, Ramkumar

AU - Kennelly, Samantha A

AU - Rodriguez, Deborah J.

AU - Harki, Daniel A.

N1 - Funding Information: We gratefully acknowledge the NIH (P01-CA234228) and the University of Minnesota for nancial support. Mass spectrometry was performed at the University of Minnesota Masonic Cancer Center Analytical Biochemistry Core Facility, which is supported by the NIH (P30-CA77598). Publisher Copyright: © 2021 The Royal Society of Chemistry.

PY - 2021/9/14

Y1 - 2021/9/14

N2 - Remdesivir is an antiviral nucleoside phosphoramidate with activity against multiple viruses, including SARS-CoV-2. To enable studies of viral polymerases with RNA containing remdesivir, we report an efficient synthesis of a phosphoramidite of GS-441524, the nucleoside precursor of remdesivir, and its incorporation into RNA using automated solid-phase RNA synthesis.

AB - Remdesivir is an antiviral nucleoside phosphoramidate with activity against multiple viruses, including SARS-CoV-2. To enable studies of viral polymerases with RNA containing remdesivir, we report an efficient synthesis of a phosphoramidite of GS-441524, the nucleoside precursor of remdesivir, and its incorporation into RNA using automated solid-phase RNA synthesis.

UR - http://www.scopus.com/inward/record.url?scp=85119886161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85119886161&partnerID=8YFLogxK

U2 - 10.1039/d1ra06589k

DO - 10.1039/d1ra06589k

M3 - Article

C2 - 35496844

AN - SCOPUS:85119886161

SN - 2046-2069

VL - 11

SP - 31373

EP - 31376

JO - RSC Advances

JF - RSC Advances

IS - 50

ER -

An efficient synthesis of RNA containing GS-441524: The nucleoside precursor of remdesivir

Abstract

Bibliographical note

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this