An efficient protocol for preparing linear β-manno-oligosaccharides

Liangnan Cui, Xiang Wang, Chao Wang, Yue Yan, Mengshan Zhang, Kevin H. Mayo, Lin Sun, Yifa Zhou

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Linear β-manno-oligosaccharides (L-β-MOS) are widely used to investigate oligo- and poly-saccharide structures and mannanolytic enzyme activities. L-β-MOS are also being used as prebiotic agents with potential bio-active properties. In this study, we developed an efficient protocol to prepare a series of L-β-MOS by hydrolyzing cassia gum (CG) using mannanolytic enzymes (endo-1,4-β-mannanase, α-galactosidases and β-glucosidases). By using medium pressure liquid chromatography (MPLC), we purified L-β-MOS with different degrees of polymerization (DPs). HPAEC-PAD, MALDI-TOF-MS and NMR studies confirmed that these L-β-MOS species ranged from 1,4-β-D-mannobiose to 1,4-β-D-mannononaose (DP 2–9) with >95% purity. Our results provide a robust approach to preparing L-β-MOS, thus enabling L-β-MOS to be further used in the fields of chemistry, life science, and nutritional food.

Original languageEnglish (US)
Article number108895
JournalCarbohydrate Research
StatePublished - Oct 2023

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (grant number: 32201051 ), the Funds from Jilin Province Development and Reform Commission (grant number: 2022C042-8) and the Fundamental Research Funds for the Central Universities (grant number: 2412021QD015 )

Publisher Copyright:
© 2023 Elsevier Ltd


  • Cassia gum
  • Enzymatic hydrolysis
  • Manno-oligosaccharides
  • Purification

PubMed: MeSH publication types

  • Journal Article


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