An efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria

F. Serafini; F. Turroni; S. Guglielmetti; L. Gioiosa; E. Foroni; V. Sanghez; A. Bartolomucci; M. O. Motherway; P. Palanza; D. van Sinderen; M. Ventura

doi:10.1111/j.1574-6968.2012.02605.x

An efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria

F. Serafini, F. Turroni, S. Guglielmetti, L. Gioiosa, E. Foroni, V. Sanghez, A. Bartolomucci, M. O. Motherway, P. Palanza, D. van Sinderen, M. Ventura

Physiology

Research output: Contribution to journal › Review article › peer-review

23 Scopus citations

Abstract

This study describes an efficient transformation system for the introduction of plasmid DNA into Bifidobacterium bifidum PRL2010 and Bifidobacterium asteroides PRL2011, for which to the best of our knowledge no transformation data have been reported previously. The method is based on electroporation of bifidobacterial cells, which were made competent by an optimized methodology based on varying media and growth conditions. Furthermore, the transformation protocol was applied in order to design a PRL2010-derivative, which carries antibiotic resistance against chloramphenicol and which was used to monitor PRL2010 colonization in a murine model.

Original language	English (US)
Pages (from-to)	146-152
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	333
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2012.02605.x
State	Published - Aug 2012

Keywords

Bifidobacteria
Genetic manipulation
Genomics
Transformation

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10.1111/j.1574-6968.2012.02605.x

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title = "An efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria",

abstract = "This study describes an efficient transformation system for the introduction of plasmid DNA into Bifidobacterium bifidum PRL2010 and Bifidobacterium asteroides PRL2011, for which to the best of our knowledge no transformation data have been reported previously. The method is based on electroporation of bifidobacterial cells, which were made competent by an optimized methodology based on varying media and growth conditions. Furthermore, the transformation protocol was applied in order to design a PRL2010-derivative, which carries antibiotic resistance against chloramphenicol and which was used to monitor PRL2010 colonization in a murine model.",

keywords = "Bifidobacteria, Genetic manipulation, Genomics, Transformation",

author = "F. Serafini and F. Turroni and S. Guglielmetti and L. Gioiosa and E. Foroni and V. Sanghez and A. Bartolomucci and Motherway, {M. O.} and P. Palanza and {van Sinderen}, D. and M. Ventura",

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AU - Serafini, F.

AU - Turroni, F.

AU - Guglielmetti, S.

AU - Gioiosa, L.

AU - Foroni, E.

AU - Sanghez, V.

AU - Bartolomucci, A.

AU - Motherway, M. O.

AU - Palanza, P.

AU - van Sinderen, D.

AU - Ventura, M.

PY - 2012/8

Y1 - 2012/8

N2 - This study describes an efficient transformation system for the introduction of plasmid DNA into Bifidobacterium bifidum PRL2010 and Bifidobacterium asteroides PRL2011, for which to the best of our knowledge no transformation data have been reported previously. The method is based on electroporation of bifidobacterial cells, which were made competent by an optimized methodology based on varying media and growth conditions. Furthermore, the transformation protocol was applied in order to design a PRL2010-derivative, which carries antibiotic resistance against chloramphenicol and which was used to monitor PRL2010 colonization in a murine model.

AB - This study describes an efficient transformation system for the introduction of plasmid DNA into Bifidobacterium bifidum PRL2010 and Bifidobacterium asteroides PRL2011, for which to the best of our knowledge no transformation data have been reported previously. The method is based on electroporation of bifidobacterial cells, which were made competent by an optimized methodology based on varying media and growth conditions. Furthermore, the transformation protocol was applied in order to design a PRL2010-derivative, which carries antibiotic resistance against chloramphenicol and which was used to monitor PRL2010 colonization in a murine model.

KW - Bifidobacteria

KW - Genetic manipulation

KW - Genomics

KW - Transformation

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An efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria

Abstract

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