An active puromycin analog derived from a non nephrotoxic aminonucleoside

Robert Vince, Ronald G. Almquist, Clare L. Ritter, Frances N. Shirota, Herbert T. Nagasawa

Research output: Contribution to journalArticlepeer-review

Abstract

Replacement of the 5' OH group of puromycin aminonucleoside (PAN) with H resulted in the elimination of kidney toxicity associated with administration of PAN. Thus, 5' deoxy PAN was not nephrotoxic to rats under the usual criteria. The corresponding 5' deoxypuromycin derived from 5' deoxy PAN was examined in a ribosomal peptidyl transferase assay and was found to be an excellent substrate for the transpeptidation reaction with bacterial ribosomes; the Km was 0.29 mM compared to the Km for puromycin of 0.20 mM. Thus, a puromycin analog has been prepared which retains puromycin like activity at the ribosomal level, but which is capable of releasing only a non nephrotoxic aminonucleoside by enzymatic hydrolysis of the p methoxyphenylalanyl side chain.

Original languageEnglish (US)
Pages (from-to)345-349
Number of pages5
JournalUnknown Journal
Volume18
Issue number3
DOIs
StatePublished - Jan 1 1976

Fingerprint Dive into the research topics of 'An active puromycin analog derived from a non nephrotoxic aminonucleoside'. Together they form a unique fingerprint.

Cite this