Utilizing the dataset corresponding to an integrated luminosity of 2.93 fb-1 at s=3.773 GeV collected by the BESIII detector, we report the first amplitude analysis and branching fraction measurement of the D0→K-π+π0π0 decay. We investigate the substructures and determine the relative fractions and the phases among the different intermediate processes. Our results are used to provide an accurate detection efficiency and allow measurement of B(D0→K-π+π0π0)=(8.86±0.13(stat)±0.19(syst))%.
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