Amplified fragment length polymorphism to detect clonal diversity and distribution of Mycobacterium avium subspecies paratuberculosis in selected Minnesota dairy cattle

Laura A. Kiehnbaum, Alongkorn Amonsin, Scott J. Wells, Vivek Kapur

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The molecular ecology of Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease, is not well understood in the United States. In this study, a DNA fingerprinting method, amplified fragment length polymorphism (AFLP), was used to subtype the pathogen and assess the clonal diversity of MAP in Minnesota dairy herds. Fifty-six fecal culture test-positive isolates from various Minnesota counties and culture dates were analyzed in this study. The AFLP identified 11 profiles with 50% of isolates representing 1 major profile. The major profile was distributed across the state. The genetic diversity of bovine MAP clones in Minnesota based on AFLP analysis of this data appears to be relatively low.

Original languageEnglish (US)
Pages (from-to)311-315
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume17
Issue number4
DOIs
StatePublished - Jul 2005

Keywords

  • Amplified fragment length polymorphisms
  • Johne's disease

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