Amphipols from a to Z*

J. L. Popot; T. Althoff; D. Bagnard; J. L. Banéres; P. Bazzacco; E. Billon-Denis; L. J. Catoire; P. Champeil; D. Charvolin; M. J. Cocco; G. Crémel; T. Dahmane; L. M. De La Maza; C. Ebel; F. Gabel; F. Giusti; Y. Gohon; E. Goormaghtigh; E. Guittet; J. H. Kleinschmidt; W. Kühlbrandt; C. Le Bon; K. L. Martinez; M. Picard; B. Pucci; J. N. Sachs; C. Tribet; C. Van Heijenoort; F. Wien; F. Zito; M. Zoonens

doi:10.1146/annurev-biophys-042910-155219

Amphipols from a to Z*

J. L. Popot, T. Althoff, D. Bagnard, J. L. Banéres, P. Bazzacco, E. Billon-Denis, L. J. Catoire, P. Champeil, D. Charvolin, M. J. Cocco, G. Crémel, T. Dahmane, L. M. De La Maza, C. Ebel, F. Gabel, F. Giusti, Y. Gohon, E. Goormaghtigh, E. Guittet, J. H. KleinschmidtW. Kühlbrandt, C. Le Bon, K. L. Martinez, M. Picard, B. Pucci, J. N. Sachs, C. Tribet, C. Van Heijenoort, F. Wien, F. Zito, M. Zoonens

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

212 Scopus citations

Abstract

Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.

Original language	English (US)
Pages (from-to)	379-408
Number of pages	30
Journal	Annual Review of Biophysics
Volume	40
Issue number	1
DOIs	https://doi.org/10.1146/annurev-biophys-042910-155219
State	Published - Jun 9 2011

Keywords

amphipathic polymers
membrane biochemistry
membrane biophysics
membrane proteins

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10.1146/annurev-biophys-042910-155219

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Popot, J. L., Althoff, T., Bagnard, D., Banéres, J. L., Bazzacco, P., Billon-Denis, E., Catoire, L. J., Champeil, P., Charvolin, D., Cocco, M. J., Crémel, G., Dahmane, T., De La Maza, L. M., Ebel, C., Gabel, F., Giusti, F., Gohon, Y., Goormaghtigh, E., Guittet, E., ... Zoonens, M. (2011). Amphipols from a to Z*. Annual Review of Biophysics, 40(1), 379-408. https://doi.org/10.1146/annurev-biophys-042910-155219

Popot, JL, Althoff, T, Bagnard, D, Banéres, JL, Bazzacco, P, Billon-Denis, E, Catoire, LJ, Champeil, P, Charvolin, D, Cocco, MJ, Crémel, G, Dahmane, T, De La Maza, LM, Ebel, C, Gabel, F, Giusti, F, Gohon, Y, Goormaghtigh, E, Guittet, E, Kleinschmidt, JH, Kühlbrandt, W, Le Bon, C, Martinez, KL, Picard, M, Pucci, B, Sachs, JN, Tribet, C, Van Heijenoort, C, Wien, F, Zito, F & Zoonens, M 2011, 'Amphipols from a to Z*', Annual Review of Biophysics, vol. 40, no. 1, pp. 379-408. https://doi.org/10.1146/annurev-biophys-042910-155219

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abstract = "Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.",

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AU - Popot, J. L.

AU - Althoff, T.

AU - Bagnard, D.

AU - Banéres, J. L.

AU - Bazzacco, P.

AU - Billon-Denis, E.

AU - Catoire, L. J.

AU - Champeil, P.

AU - Charvolin, D.

AU - Cocco, M. J.

AU - Crémel, G.

AU - Dahmane, T.

AU - De La Maza, L. M.

AU - Ebel, C.

AU - Gabel, F.

AU - Giusti, F.

AU - Gohon, Y.

AU - Goormaghtigh, E.

AU - Guittet, E.

AU - Kleinschmidt, J. H.

AU - Kühlbrandt, W.

AU - Le Bon, C.

AU - Martinez, K. L.

AU - Picard, M.

AU - Pucci, B.

AU - Sachs, J. N.

AU - Tribet, C.

AU - Van Heijenoort, C.

AU - Wien, F.

AU - Zito, F.

AU - Zoonens, M.

PY - 2011/6/9

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N2 - Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.

AB - Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.

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ER -

Amphipols from a to Z*

Abstract

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