Bhatt et al. use cryo-EM to reveal the organization of N-BAR protein endophilin B1 assembled on tubulated membranes. Interactions between N-terminal amphipathic motif H0 and membrane lipids direct helical assembly of dimers. Membrane binding and subsequent remodeling is auto-inhibited, mediated by H0-SH3 domain interactions, and influenced by membrane lipid composition.
Bibliographical noteFunding Information:
We thank Drs. Andrew Kehr and Hong-Gang Wang for generously gifting us HRV-3C protease and endophilin B1 plasmids. This work was funded by The Hormel Foundation and the Paint the Town Pink Foundation for Breast Cancer Research .
- BAR proteins
- amphipathic helices
- membrane remodeling