Amino Surface Modification and Fluorescent Labelling of Porous Hollow Organosilica Particles: Optimization and Characterization

Mohammed A. Al-khafaji, Anikó Gaál, Bálint Jezsó, Judith Mihály, Zoltán Varga

Research output: Contribution to journalArticlepeer-review

Abstract

Surface modification of silica nanoparticles with organic functional groups while main-taining colloidal stability remains a synthetic challenge. This work aimed to prepare highly dispersed porous hollow organosilica particles (pHOPs) with amino surface modification. The amino-surface modification of pHOPs was carried out with 3-aminopropyl(diethoxy)methylsilane (APDEMS) under various reaction parameters, and the optimal pHOP-NH2 sample was selected and labelled with fluorescein isothiocyanate (FITC) to achieve fluorescent pHOPs (F-HOPs). The prepared pHOPs were thoroughly characterized by transmission electron microscopy, dynamic light scattering, FT-IR, UV-Vis and fluorescence spectroscopies, and microfluidic resistive pulse sensing. The optimal amino surface modification of pHOPs with APDEMS was at pH 10.2, at 60 °C temperature with 10 min reaction time. The positive Zeta potential of pHOP-NH2 in an acidic environment and the appearance of vibrations characteristic to the surface amino groups on the FT-IR spectra prove the successful surface modification. A red-shift in the absorbance spectrum and the appearance of bands characteristic to secondary amines in the FTIR spectrum of F-HOP confirmed the covalent attachment of FITC to pHOP-NH2. This study provides a step-by-step synthetic optimization and characterization of fluorescently labelled organosilica particles to enhance their optical properties and extend their applications.

Original languageEnglish (US)
Article number2696
JournalMaterials
Volume15
Issue number7
DOIs
StatePublished - Apr 1 2022

Bibliographical note

Funding Information:
Funding: This research was partially funded by the EMPIR programme co‐financed by the Partici‐ pating States and from the European Union’s Horizon 2020 research and innovation programme, grant number 18HLT01 METVES II and by the National Research, Development and Innovation Office, grant number K131594. ZV was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences.

Funding Information:
This research was partially funded by the EMPIR programme co-financed by the Partici-pating States and from the European Union?s Horizon 2020 research and innovation programme, grant number 18HLT01 METVES II and by the National Research, Development and Innovation Office, grant number K131594. ZV was supported by the J?nos Bolyai Research Scholarship of the Hungarian Academy of Sciences.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • aminopropyl surface modification
  • fluorescent labelling
  • hollow silica particles

PubMed: MeSH publication types

  • Journal Article

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