Alternative Secondary Structures in the Phage G4 Origin of the Complementary DNA Strand Synthesis: Effects of NaCl Concentration on the Bleomycin-DNA Interaction

Kazumitsu Ueda, Hiroshi Hiasa, So Takebe, Hiroshi Sakai, Tohru Komano

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of NaCl concentration on bleomycin-induced cleavages of single-strand and double-strand DNA fragments containing the phage G4 origin of complementary DNA Strand synthesis were investigated. It was found that bleomycin could be used as a reagent to analyze secondary and tertiary structures and subtle changes of DNA structures. The effects of NaCl concentration on cleavages of single-stranded DNA were distinct at every target site, indicating that the diversity of topolotical properties of DNA might change the selectivity of the bleomycin-induced DNA cleavage. These results showed alternative secondary structures within and close to the G4 origin of complementary DNA Strand synthesis.

Original languageEnglish (US)
Pages (from-to)394-398
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume56
Issue number3
DOIs
StatePublished - 1992

Bibliographical note

Funding Information:
Acknowledgments. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan

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