Alterations in human glomerular epithelial cells interacting with nonenzymatically glycosylated matrix

Uma Krishnamurti, Eric Rondeau, Jean Daniel Sraer, Alfred F. Michael, Effie C. Tsilibary

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

The glomerular epithelial cells and the glomerular basement membrane are important constituents of the permselective barrier in the kidney. These are affected in diabetic nephropathy, one of the long-term complications in diabetic patients. Nonenzymatic glycosylation resulting in the accumulation of advanced glycosylation end products correlates with the development of long-term complications in diabetes. The interaction of cells with extracellular matrix proteins plays a critical role in a variety of biological processes. Recent studies show that cell-matrix interactions mediated by integrins can transduce biochemical signals to the cell interior and regulate cell behavior. In this paper we demonstrate that interactions of human glomerular epithelial cells with a nonenzymatically glycated matrix are altered with defective cell spreading, reduced phosphorylation of focal adhesion kinase and reduced activity of mitogen-activated protein kinase. These data suggest that matrix glycation interferes with normal cell-matrix interactions and intracellular signaling that can potentially result in differential gene expression contributing to the changes seen in diabetic nephropathy.

Original languageEnglish (US)
Pages (from-to)27966-27970
Number of pages5
JournalJournal of Biological Chemistry
Volume272
Issue number44
DOIs
StatePublished - Oct 31 1997

Fingerprint

Dive into the research topics of 'Alterations in human glomerular epithelial cells interacting with nonenzymatically glycosylated matrix'. Together they form a unique fingerprint.

Cite this