Alterations in colonic smooth muscle function in cats with idiopathic megacolon

Robert J. Washabau, Ilse H. Stalis

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective - To determine whether colonie smooth muscle dysfunction is involved in the pathogenesis of idiopathic megacolon in cats. Design - In vitro smooth muscle mechanical measurements. Animals - Colon from healthy cats and cats with idiopathic megacolon Procedure - Colonic smooth muscle strips were suspended in physiologic buffer solution, attached to isometric force transducers, and contracted with acetylcholine (ACh; 10-9 to 10-4M), substance P (SP; 10-10 to 10-6M), cholecystokinm (CCK; 10-11 to 10- 6M), potassium chloride (KCl; 10 to 80 mM), or electrical field stimulation (EFS; 25 V. 1 to 30 Hz, 0.5-millisecond duration). Isometric stress responses were compared with those obtained from healthy controls. Colonic smooth muscle strips were also evaluated histotogically for neuronal and smooth muscle cell morphology. Results - Passive isometric stress was not altered, but the active isometric stress responses of megacolon smooth muscle to ACh, SP, CCK, KCl, and EFS were significantly (P < 0 05) diminished, compared with healthy controls. Differences were observed in longitudinal and circular smooth muscle from proximal and distal portions of the colon. Histologic evaluation revealed few abnormalities of smooth muscle cells or of myenteric or submucosal plexus neurons. The contractile response of megacolon smooth muscle to EFS, and the inhibition of this response by tetrodotoxin, suggest that myenteric and submucosal plexus neurons in megacolon smooth muscle are functional. Conclusions - Idiopathic megacolon is a generalized dysfunction of colonic smooth muscle in cats. The diminished isometric stress responses to receptor occupancy (ACh, SP, and CCK) and membrane depolarization (KCl) further suggest that the disorder involves disturbance in the activation of smooth muscle myofilaments.

Original languageEnglish (US)
Pages (from-to)580-587
Number of pages8
JournalAmerican journal of veterinary research
Volume57
Issue number4
StatePublished - Apr 1 1996

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megacolon
Megacolon
smooth muscle
Smooth Muscle
Cats
cats
Submucous Plexus
Myenteric Plexus
stress response
plexus
Smooth Muscle Myocytes
Colon
myocytes
colon
Neurons
neurons
Potassium Chloride
Myofibrils
Tetrodotoxin
Cholinergic Receptors

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Alterations in colonic smooth muscle function in cats with idiopathic megacolon. / Washabau, Robert J.; Stalis, Ilse H.

In: American journal of veterinary research, Vol. 57, No. 4, 01.04.1996, p. 580-587.

Research output: Contribution to journalArticle

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abstract = "Objective - To determine whether colonie smooth muscle dysfunction is involved in the pathogenesis of idiopathic megacolon in cats. Design - In vitro smooth muscle mechanical measurements. Animals - Colon from healthy cats and cats with idiopathic megacolon Procedure - Colonic smooth muscle strips were suspended in physiologic buffer solution, attached to isometric force transducers, and contracted with acetylcholine (ACh; 10-9 to 10-4M), substance P (SP; 10-10 to 10-6M), cholecystokinm (CCK; 10-11 to 10- 6M), potassium chloride (KCl; 10 to 80 mM), or electrical field stimulation (EFS; 25 V. 1 to 30 Hz, 0.5-millisecond duration). Isometric stress responses were compared with those obtained from healthy controls. Colonic smooth muscle strips were also evaluated histotogically for neuronal and smooth muscle cell morphology. Results - Passive isometric stress was not altered, but the active isometric stress responses of megacolon smooth muscle to ACh, SP, CCK, KCl, and EFS were significantly (P < 0 05) diminished, compared with healthy controls. Differences were observed in longitudinal and circular smooth muscle from proximal and distal portions of the colon. Histologic evaluation revealed few abnormalities of smooth muscle cells or of myenteric or submucosal plexus neurons. The contractile response of megacolon smooth muscle to EFS, and the inhibition of this response by tetrodotoxin, suggest that myenteric and submucosal plexus neurons in megacolon smooth muscle are functional. Conclusions - Idiopathic megacolon is a generalized dysfunction of colonic smooth muscle in cats. The diminished isometric stress responses to receptor occupancy (ACh, SP, and CCK) and membrane depolarization (KCl) further suggest that the disorder involves disturbance in the activation of smooth muscle myofilaments.",
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N2 - Objective - To determine whether colonie smooth muscle dysfunction is involved in the pathogenesis of idiopathic megacolon in cats. Design - In vitro smooth muscle mechanical measurements. Animals - Colon from healthy cats and cats with idiopathic megacolon Procedure - Colonic smooth muscle strips were suspended in physiologic buffer solution, attached to isometric force transducers, and contracted with acetylcholine (ACh; 10-9 to 10-4M), substance P (SP; 10-10 to 10-6M), cholecystokinm (CCK; 10-11 to 10- 6M), potassium chloride (KCl; 10 to 80 mM), or electrical field stimulation (EFS; 25 V. 1 to 30 Hz, 0.5-millisecond duration). Isometric stress responses were compared with those obtained from healthy controls. Colonic smooth muscle strips were also evaluated histotogically for neuronal and smooth muscle cell morphology. Results - Passive isometric stress was not altered, but the active isometric stress responses of megacolon smooth muscle to ACh, SP, CCK, KCl, and EFS were significantly (P < 0 05) diminished, compared with healthy controls. Differences were observed in longitudinal and circular smooth muscle from proximal and distal portions of the colon. Histologic evaluation revealed few abnormalities of smooth muscle cells or of myenteric or submucosal plexus neurons. The contractile response of megacolon smooth muscle to EFS, and the inhibition of this response by tetrodotoxin, suggest that myenteric and submucosal plexus neurons in megacolon smooth muscle are functional. Conclusions - Idiopathic megacolon is a generalized dysfunction of colonic smooth muscle in cats. The diminished isometric stress responses to receptor occupancy (ACh, SP, and CCK) and membrane depolarization (KCl) further suggest that the disorder involves disturbance in the activation of smooth muscle myofilaments.

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