Allergenome characterization of the mosquito Aedes aegypti

J. F. Cantillo, L. Puerta, P. Puchalska, S. Lafosse-Marin, J. L. Subiza, E. Fernández-Caldas

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Background: Saliva and muscle-derived mosquito allergens have been purified and characterized. However, the complete set of allergens remains to be elucidated. In this study, we identified and characterized IgE-binding proteins from the mosquito species Aedes aegypti. Methods: Serum was obtained from 15 allergic individuals with asthma and/or rhinitis and sensitized to mosquito. IgE binding was determined by ELISA. Total proteins from freeze-dried bodies of A. aegypti were extracted and IgE-reactive proteins were identified by 2D gel electrophoresis, followed by Western blot with pooled or individual sera. IgE-reactive spots were further characterized by mass spectrometry. Results: Twenty-five IgE-reactive spots were identified, corresponding to 10 different proteins, some of which appeared as different variants or isoforms. Heat-shock cognate 70 (HSC-70) and tropomyosin showed IgE reactivity with 60% of the sera, lysosomal aspartic protease, and “AAEL006070-PA” (Uniprot: Q177P3) with 40% and the other proteins with <33.3% of the sera. Different variants or isoforms of tropomyosin, arginine or creatine kinase, glyceraldehyde-3-phosphate dehydrogenase (GPDH), calcium-binding protein, and phosphoglycerate mutase were also identified. The mixture of three allergens (Aed a 6, Aed a 8, and Aed a 10) seems to identify more than 80% of A. aegypti-sensitized individuals, indicating that these allergens should be considered when designing of improved mosquito allergy diagnostic tools. Conclusions: The newly identified allergens may play a role in the pathophysiology of mosquito allergy in the tropics, and some of them might be important arthropod-related proteins involved in cross-reactivity between A. aegypti and other allergenic arthropods.

Original languageEnglish (US)
Pages (from-to)1499-1509
Number of pages11
JournalAllergy: European Journal of Allergy and Clinical Immunology
Issue number10
StatePublished - Oct 2017
Externally publishedYes

Bibliographical note

Funding Information:
Jos? Fernando Cantillo is recipient of a fellowship from Colciencias, Colombia. Mass spectrometry analysis was performed in Proteomics Platform CIC bioGUNE, Derio, Spain. This study has also been partially funded by Inmunotek SL.

Publisher Copyright:
© 2017 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.

Copyright 2018 Elsevier B.V., All rights reserved.


  • Aedes aegypti
  • allergen
  • allergenome
  • insect allergy
  • mosquito

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