Allergenome characterization of the mosquito Aedes aegypti

J. F. Cantillo, L. Puerta, P. Puchalska, S. Lafosse-Marin, J. L. Subiza, E. Fernández-Caldas

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4 Scopus citations


Background: Saliva and muscle-derived mosquito allergens have been purified and characterized. However, the complete set of allergens remains to be elucidated. In this study, we identified and characterized IgE-binding proteins from the mosquito species Aedes aegypti. Methods: Serum was obtained from 15 allergic individuals with asthma and/or rhinitis and sensitized to mosquito. IgE binding was determined by ELISA. Total proteins from freeze-dried bodies of A. aegypti were extracted and IgE-reactive proteins were identified by 2D gel electrophoresis, followed by Western blot with pooled or individual sera. IgE-reactive spots were further characterized by mass spectrometry. Results: Twenty-five IgE-reactive spots were identified, corresponding to 10 different proteins, some of which appeared as different variants or isoforms. Heat-shock cognate 70 (HSC-70) and tropomyosin showed IgE reactivity with 60% of the sera, lysosomal aspartic protease, and “AAEL006070-PA” (Uniprot: Q177P3) with 40% and the other proteins with <33.3% of the sera. Different variants or isoforms of tropomyosin, arginine or creatine kinase, glyceraldehyde-3-phosphate dehydrogenase (GPDH), calcium-binding protein, and phosphoglycerate mutase were also identified. The mixture of three allergens (Aed a 6, Aed a 8, and Aed a 10) seems to identify more than 80% of A. aegypti-sensitized individuals, indicating that these allergens should be considered when designing of improved mosquito allergy diagnostic tools. Conclusions: The newly identified allergens may play a role in the pathophysiology of mosquito allergy in the tropics, and some of them might be important arthropod-related proteins involved in cross-reactivity between A. aegypti and other allergenic arthropods.

Original languageEnglish (US)
Pages (from-to)1499-1509
Number of pages11
JournalAllergy: European Journal of Allergy and Clinical Immunology
Issue number10
StatePublished - Oct 2017
Externally publishedYes



  • Aedes aegypti
  • allergen
  • allergenome
  • insect allergy
  • mosquito

Cite this

Cantillo, J. F., Puerta, L., Puchalska, P., Lafosse-Marin, S., Subiza, J. L., & Fernández-Caldas, E. (2017). Allergenome characterization of the mosquito Aedes aegypti. Allergy: European Journal of Allergy and Clinical Immunology, 72(10), 1499-1509.