Projects per year
Abstract
Collagen matrices are one form of artificial tissue that has applications in biomimetic organs or tumors, and in fundamental biology. Anatomical organs and tissues are often composed of aligned collagen, and in this study cross-linking nickel magnetic nanowires (MNWs) to collagen allowed a one-step bi-directional alignment of the collagen matrices when processed in a uniform magnetic field. These matrices were analyzed by differential interference contrast (DIC) microscopy, scanning electron microscopy (SEM) and polarized transmittance. The bi-directional alignment was also confirmed by plated, stained arachnoid cells from the blood-brain-barrier (BBB). Arachnoid cells are morphologically sensitive to their extracellular matrix (ECM) environment, and in this study, they were observed to spider out in two distinct directions as predicted by microscopy and transmittance. In fact, MNW-collagen matrices plated with arachnoid-cells are promising for future studies of artificial BBBs. Other cells (here osteosarcoma) have been observed to internalize MNWs, which leads to the possibility of barcoding matrices and cells with distinct signatures, pending a magnetic readout technique. To this aim, mixtures of two different MNW populations were analyzed using first order reversal curves (FORC), and the relative concentrations of the two populations were correctly estimated with negligible error for ratios of 1: 23 and only 7% error for ratios of 1: 115. Together, these studies open a path for magnetic identification of artificial tissues where distinct magnetic labels on matrices and in cells combine for a unique fingerprint.
Original language | English (US) |
---|---|
Pages (from-to) | 176-181 |
Number of pages | 6 |
Journal | Journal of Magnetism and Magnetic Materials |
Volume | 459 |
DOIs | |
State | Published - Aug 1 2018 |
Bibliographical note
Publisher Copyright:© 2017
Keywords
- Arachnoid cells
- Blood-brain-barrier
- Collagen
- FORC
- Magnetic alignment
- Magnetic nanowires
- Nano barcodes
- Osteosarcoma
MRSEC Support
- Shared
Fingerprint
Dive into the research topics of 'Alignment of collagen matrices using magnetic nanowires and magnetic barcode readout using first order reversal curves (FORC) (invited)'. Together they form a unique fingerprint.Projects
- 3 Finished
-
-
University of Minnesota MRSEC (DMR-1420013)
Lodge, T. P. (PI)
11/1/14 → 10/31/20
Project: Research project
-
University Assets
-
Olympus Fluoview FV1000 IX2 Inverted Confocal with FLIM Detector
University Imaging CentersEquipment/facility: Equipment
-
University Imaging Centers
Sanders, M. A. (Program Director)
University Imaging CentersEquipment/facility: Facility