Alignment of collagen matrices using magnetic nanowires and magnetic barcode readout using first order reversal curves (FORC) (invited)

Anirudh Sharma, Michael D. DiVito, Daniel E. Shore, Andrew D. Block, Katie Pollock, Peter Solheid, Joshua M. Feinberg, Jaime Modiano, Cornelius H. Lam, Allison Hubel, Bethanie J.H. Stadler

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Collagen matrices are one form of artificial tissue that has applications in biomimetic organs or tumors, and in fundamental biology. Anatomical organs and tissues are often composed of aligned collagen, and in this study cross-linking nickel magnetic nanowires (MNWs) to collagen allowed a one-step bi-directional alignment of the collagen matrices when processed in a uniform magnetic field. These matrices were analyzed by differential interference contrast (DIC) microscopy, scanning electron microscopy (SEM) and polarized transmittance. The bi-directional alignment was also confirmed by plated, stained arachnoid cells from the blood-brain-barrier (BBB). Arachnoid cells are morphologically sensitive to their extracellular matrix (ECM) environment, and in this study, they were observed to spider out in two distinct directions as predicted by microscopy and transmittance. In fact, MNW-collagen matrices plated with arachnoid-cells are promising for future studies of artificial BBBs. Other cells (here osteosarcoma) have been observed to internalize MNWs, which leads to the possibility of barcoding matrices and cells with distinct signatures, pending a magnetic readout technique. To this aim, mixtures of two different MNW populations were analyzed using first order reversal curves (FORC), and the relative concentrations of the two populations were correctly estimated with negligible error for ratios of 1: 23 and only 7% error for ratios of 1: 115. Together, these studies open a path for magnetic identification of artificial tissues where distinct magnetic labels on matrices and in cells combine for a unique fingerprint.

Original languageEnglish (US)
Pages (from-to)176-181
Number of pages6
JournalJournal of Magnetism and Magnetic Materials
Volume459
DOIs
StatePublished - Aug 1 2018

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Collagen
Nanowires
readout
nanowires
alignment
curves
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Microscopic examination
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transmittance
Biomimetics
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Keywords

  • Arachnoid cells
  • Blood-brain-barrier
  • Collagen
  • FORC
  • Magnetic alignment
  • Magnetic nanowires
  • Nano barcodes
  • Osteosarcoma

How much support was provided by MRSEC?

  • Shared

Reporting period for MRSEC

  • Period 5

Cite this

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title = "Alignment of collagen matrices using magnetic nanowires and magnetic barcode readout using first order reversal curves (FORC) (invited)",
abstract = "Collagen matrices are one form of artificial tissue that has applications in biomimetic organs or tumors, and in fundamental biology. Anatomical organs and tissues are often composed of aligned collagen, and in this study cross-linking nickel magnetic nanowires (MNWs) to collagen allowed a one-step bi-directional alignment of the collagen matrices when processed in a uniform magnetic field. These matrices were analyzed by differential interference contrast (DIC) microscopy, scanning electron microscopy (SEM) and polarized transmittance. The bi-directional alignment was also confirmed by plated, stained arachnoid cells from the blood-brain-barrier (BBB). Arachnoid cells are morphologically sensitive to their extracellular matrix (ECM) environment, and in this study, they were observed to spider out in two distinct directions as predicted by microscopy and transmittance. In fact, MNW-collagen matrices plated with arachnoid-cells are promising for future studies of artificial BBBs. Other cells (here osteosarcoma) have been observed to internalize MNWs, which leads to the possibility of barcoding matrices and cells with distinct signatures, pending a magnetic readout technique. To this aim, mixtures of two different MNW populations were analyzed using first order reversal curves (FORC), and the relative concentrations of the two populations were correctly estimated with negligible error for ratios of 1: 23 and only 7{\%} error for ratios of 1: 115. Together, these studies open a path for magnetic identification of artificial tissues where distinct magnetic labels on matrices and in cells combine for a unique fingerprint.",
keywords = "Arachnoid cells, Blood-brain-barrier, Collagen, FORC, Magnetic alignment, Magnetic nanowires, Nano barcodes, Osteosarcoma",
author = "Anirudh Sharma and DiVito, {Michael D.} and Shore, {Daniel E.} and Block, {Andrew D.} and Katie Pollock and Peter Solheid and Feinberg, {Joshua M.} and Jaime Modiano and Lam, {Cornelius H.} and Allison Hubel and Stadler, {Bethanie J.H.}",
year = "2018",
month = "8",
day = "1",
doi = "10.1016/j.jmmm.2017.11.035",
language = "English (US)",
volume = "459",
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TY - JOUR

T1 - Alignment of collagen matrices using magnetic nanowires and magnetic barcode readout using first order reversal curves (FORC) (invited)

AU - Sharma, Anirudh

AU - DiVito, Michael D.

AU - Shore, Daniel E.

AU - Block, Andrew D.

AU - Pollock, Katie

AU - Solheid, Peter

AU - Feinberg, Joshua M.

AU - Modiano, Jaime

AU - Lam, Cornelius H.

AU - Hubel, Allison

AU - Stadler, Bethanie J.H.

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Collagen matrices are one form of artificial tissue that has applications in biomimetic organs or tumors, and in fundamental biology. Anatomical organs and tissues are often composed of aligned collagen, and in this study cross-linking nickel magnetic nanowires (MNWs) to collagen allowed a one-step bi-directional alignment of the collagen matrices when processed in a uniform magnetic field. These matrices were analyzed by differential interference contrast (DIC) microscopy, scanning electron microscopy (SEM) and polarized transmittance. The bi-directional alignment was also confirmed by plated, stained arachnoid cells from the blood-brain-barrier (BBB). Arachnoid cells are morphologically sensitive to their extracellular matrix (ECM) environment, and in this study, they were observed to spider out in two distinct directions as predicted by microscopy and transmittance. In fact, MNW-collagen matrices plated with arachnoid-cells are promising for future studies of artificial BBBs. Other cells (here osteosarcoma) have been observed to internalize MNWs, which leads to the possibility of barcoding matrices and cells with distinct signatures, pending a magnetic readout technique. To this aim, mixtures of two different MNW populations were analyzed using first order reversal curves (FORC), and the relative concentrations of the two populations were correctly estimated with negligible error for ratios of 1: 23 and only 7% error for ratios of 1: 115. Together, these studies open a path for magnetic identification of artificial tissues where distinct magnetic labels on matrices and in cells combine for a unique fingerprint.

AB - Collagen matrices are one form of artificial tissue that has applications in biomimetic organs or tumors, and in fundamental biology. Anatomical organs and tissues are often composed of aligned collagen, and in this study cross-linking nickel magnetic nanowires (MNWs) to collagen allowed a one-step bi-directional alignment of the collagen matrices when processed in a uniform magnetic field. These matrices were analyzed by differential interference contrast (DIC) microscopy, scanning electron microscopy (SEM) and polarized transmittance. The bi-directional alignment was also confirmed by plated, stained arachnoid cells from the blood-brain-barrier (BBB). Arachnoid cells are morphologically sensitive to their extracellular matrix (ECM) environment, and in this study, they were observed to spider out in two distinct directions as predicted by microscopy and transmittance. In fact, MNW-collagen matrices plated with arachnoid-cells are promising for future studies of artificial BBBs. Other cells (here osteosarcoma) have been observed to internalize MNWs, which leads to the possibility of barcoding matrices and cells with distinct signatures, pending a magnetic readout technique. To this aim, mixtures of two different MNW populations were analyzed using first order reversal curves (FORC), and the relative concentrations of the two populations were correctly estimated with negligible error for ratios of 1: 23 and only 7% error for ratios of 1: 115. Together, these studies open a path for magnetic identification of artificial tissues where distinct magnetic labels on matrices and in cells combine for a unique fingerprint.

KW - Arachnoid cells

KW - Blood-brain-barrier

KW - Collagen

KW - FORC

KW - Magnetic alignment

KW - Magnetic nanowires

KW - Nano barcodes

KW - Osteosarcoma

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U2 - 10.1016/j.jmmm.2017.11.035

DO - 10.1016/j.jmmm.2017.11.035

M3 - Article

AN - SCOPUS:85034829265

VL - 459

SP - 176

EP - 181

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

SN - 0304-8853

ER -