Alignment maps of tissues: II. Fast harmonic analysis for imaging

Theodore T. Tower, Robert T. Tranquillo

Research output: Contribution to journalArticlepeer-review

81 Scopus citations


A methodology for generating polarized light retardation and alignment direction images is presented. A rotated quarter-wave plate changes the linear polarized light to a polarized probe with various degrees of ellipticity by which samples are imaged with the use of a circular analyzer. A harmonic representation of image intensity allows simple analysis, requiring only simple image operations and realizing four orders-of-magnitude computational savings for strongly aligned tissues, where linear birefringence is the dominant optical property. The method is demonstrated for a porcine heart valve leaflet.

Original languageEnglish (US)
Pages (from-to)2964-2971
Number of pages8
JournalBiophysical journal
Issue number5
StatePublished - 2001

Bibliographical note

Funding Information:
This work was supported by the Engineering Research Centers Program (EEC-8721551) of the National Science Foundation.


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