Abstract
A methodology for generating polarized light retardation and alignment direction images is presented. A rotated quarter-wave plate changes the linear polarized light to a polarized probe with various degrees of ellipticity by which samples are imaged with the use of a circular analyzer. A harmonic representation of image intensity allows simple analysis, requiring only simple image operations and realizing four orders-of-magnitude computational savings for strongly aligned tissues, where linear birefringence is the dominant optical property. The method is demonstrated for a porcine heart valve leaflet.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2964-2971 |
| Number of pages | 8 |
| Journal | Biophysical journal |
| Volume | 81 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2001 |
Bibliographical note
Funding Information:This work was supported by the Engineering Research Centers Program (EEC-8721551) of the National Science Foundation.