Aligned human microvessels formed in 3D fibrin gel by constraint of gel contraction

Kristen T. Morin, Annie O. Smith, George E. Davis, Robert T. Tranquillo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This study aimed to form microvessels in fibrin gels, which is of interest both for studying the fundamental cell-matrix interactions as well as for tissue engineering purposes, and to align the microvessels, which would provide natural inlet and outlet sides for perfusion. The data reported here demonstrate the formation of highly interconnected microvessels in fibrin gel under defined medium conditions and the ability to align them using two methods, both of which involved anchoring the gel at both ends to constrain the cell-induced compaction. The first method used only defined medium and resulted in moderate alignment. The second method used defined and serum-containing media sequentially to achieve high levels of microvessel alignment.

Original languageEnglish (US)
Pages (from-to)12-22
Number of pages11
JournalMicrovascular Research
Volume90
DOIs
StatePublished - Nov 1 2013

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Microvessels
Fibrin
Gels
Tissue engineering
Compaction
Tissue Engineering
Cell Communication
Perfusion
Serum

Cite this

Aligned human microvessels formed in 3D fibrin gel by constraint of gel contraction. / Morin, Kristen T.; Smith, Annie O.; Davis, George E.; Tranquillo, Robert T.

In: Microvascular Research, Vol. 90, 01.11.2013, p. 12-22.

Research output: Contribution to journalArticle

Morin, Kristen T. ; Smith, Annie O. ; Davis, George E. ; Tranquillo, Robert T. / Aligned human microvessels formed in 3D fibrin gel by constraint of gel contraction. In: Microvascular Research. 2013 ; Vol. 90. pp. 12-22.
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