Aligned human microvessels formed in 3D fibrin gel by constraint of gel contraction

Kristen T. Morin, Annie O. Smith, George E. Davis, Robert T. Tranquillo

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23 Scopus citations

Abstract

This study aimed to form microvessels in fibrin gels, which is of interest both for studying the fundamental cell-matrix interactions as well as for tissue engineering purposes, and to align the microvessels, which would provide natural inlet and outlet sides for perfusion. The data reported here demonstrate the formation of highly interconnected microvessels in fibrin gel under defined medium conditions and the ability to align them using two methods, both of which involved anchoring the gel at both ends to constrain the cell-induced compaction. The first method used only defined medium and resulted in moderate alignment. The second method used defined and serum-containing media sequentially to achieve high levels of microvessel alignment.

Original languageEnglish (US)
Pages (from-to)12-22
Number of pages11
JournalMicrovascular Research
Volume90
DOIs
StatePublished - Nov 2013

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health [ R01 HL108670 to RTT]; and the American Heart Association [ 11PRE7610056 to KTM].

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