Aligned collagen is a prognostic signature for survival in human breast carcinoma

Matthew W. Conklin, Jens C. Eickhoff, Kristin M. Riching, Carolyn A. Pehlke, Kevin W. Eliceiri, Paolo P. Provenzano, Andreas Friedl, Patricia J. Keely

Research output: Contribution to journalArticlepeer-review

617 Scopus citations

Abstract

Evidence for the potent influence of stromal organization and function on invasion and metastasis of breast tumors is ever growing. We have performed a rigorous examination of the relationship of a tumor-associated collagen signature-3 (TACS-3) to the longterm survival rate of human patients. TACS-3 is characterized by bundles of straightened and aligned collagen fibers that are oriented perpendicular to the tumor boundary. An evaluation of TACS-3 was performed in biopsied tissue sections from 196 patients by second harmonic generation imaging of the back-scattered signal generated by collagen. Univariate analysis of a Cox proportional hazard model demonstrated that the presence of TACS-3 was associated with poor disease-specific and disease-free survival, resulting in hazard ratios between 3.0 and 3.9. Furthermore, TACS-3 was confirmed to be an independent prognostic indicator regardless of tumor grade and size, estrogen or progesterone receptor status, human epidermal growth factor receptor-2 status, node status, and tumor subtype. Interestingly, TACS-3 was positively correlated to expression of stromal syndecan-1, a receptor for several extracellular matrix proteins including collagens. Because of the strong statistical evidence for poor survival in patients with TACS, and because the assessment can be performed in routine histopathological samples imaged via second harmonic generation or using picrosirius, we propose that quantifying collagen alignment is a viable, novel paradigm for the prediction of human breast cancer survival.

Original languageEnglish (US)
Pages (from-to)1221-1232
Number of pages12
JournalAmerican Journal of Pathology
Volume178
Issue number3
DOIs
StatePublished - Mar 2011

Bibliographical note

Funding Information:
This work was supported by a grant from the Mary Kay Ash Charitable Foundation (P.J.K.), a Coulter Foundation Award (K.W.E and P.J.K.), grants from the National Institutes of Health (NIH) RO1 CA114462 to P.J.K., NIH RO1 CA142833 to P.J.K., NIH R01 EB000184 award to K.W.E., a DOD-CDMRP/BCRP W81XWH-04-1-042 award to P.P.P., and an NIH 2RO1 CA107012-06 award to A.F.

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