Alcohol Effects on Colon Epithelium are Time-Dependent

Faraz Bishehsari, Lijuan Zhang, Robin M. Voigt, Natalie Maltby, Bita Semsarieh, Eyas Zorub, Maliha Shaikh, Sherry Wilber, Andrew R. Armstrong, Seyed Sina Mirbagheri, Nailliw Z. Preite, Peter Song, Alessia Stornetta, Silvia Balbo, Christopher B. Forsyth, Ali Keshavarzian

Research output: Contribution to journalArticle

Abstract

Background: Alcohol intake increases the risk of developing colon cancer. Circadian disruption promotes alcohol's effect on colon carcinogenesis through unknown mechanisms. Alcohol's metabolites induce DNA damage, an early step in carcinogenesis. We assessed the effect of time of alcohol consumption on markers of tissue damage in the colonic epithelium. Methods: Mice were treated by alcohol or phosphate-buffered saline (PBS), at 4-hour intervals for 3 days, and their colons were analyzed for (i) proliferation (Ki67) and antiapoptosis (Bcl-2) markers, (ii) DNA damage (γ-H2AX), and (iii) the major acetaldehyde (AcH)–DNA adduct, N2-ethylidene-dG. To model circadian disruption, mice were shifted once weekly for 12 h and then were sacrificed at 4-hour intervals. Samples of mice with a dysfunctional molecular clock were analyzed. The dynamics of DNA damage repair from AcH treatment as well as role of xeroderma pigmentosum, complementation group A (XPA) in their repair were studied in vitro. Results: Proliferation and survival of colonic epithelium have daily rhythmicity. Alcohol induced colonic epithelium proliferation in a time-dependent manner, with a stronger effect during the light/rest period. Alcohol-associated DNA damage also occurred more when alcohol was given at light. Levels of DNA adduct did not vary by time, suggesting rather lower repair efficiency during the light versus dark. XPA gene expression, a key excision repair gene, was time-dependent, peaking at the beginning of the dark. XPA knockout colon epithelial cells were inefficient in repair of the DNA damage induced by alcohol's metabolite. Conclusions: Time of day of alcohol intake may be an important determinant of colon tissue damage and carcinogenicity.

Original languageEnglish (US)
Pages (from-to)1898-1908
Number of pages11
JournalAlcoholism: Clinical and Experimental Research
Volume43
Issue number9
DOIs
StatePublished - Sep 1 2019

Fingerprint

Colon
Epithelium
Alcohols
DNA Damage
Repair
DNA
Acetaldehyde
DNA Adducts
Light
DNA Repair
Metabolites
Carcinogenesis
Xeroderma Pigmentosum
Tissue
Periodicity
Alcohol Drinking
Colonic Neoplasms
Gene expression
Clocks
Epithelial Cells

Keywords

  • Alcohol
  • Circadian
  • Colon Carcinogenesis
  • Time
  • Xeroderma Pigmentosum, Complementation Group A

PubMed: MeSH publication types

  • Journal Article

Cite this

Bishehsari, F., Zhang, L., Voigt, R. M., Maltby, N., Semsarieh, B., Zorub, E., ... Keshavarzian, A. (2019). Alcohol Effects on Colon Epithelium are Time-Dependent. Alcoholism: Clinical and Experimental Research, 43(9), 1898-1908. https://doi.org/10.1111/acer.14141

Alcohol Effects on Colon Epithelium are Time-Dependent. / Bishehsari, Faraz; Zhang, Lijuan; Voigt, Robin M.; Maltby, Natalie; Semsarieh, Bita; Zorub, Eyas; Shaikh, Maliha; Wilber, Sherry; Armstrong, Andrew R.; Mirbagheri, Seyed Sina; Preite, Nailliw Z.; Song, Peter; Stornetta, Alessia; Balbo, Silvia; Forsyth, Christopher B.; Keshavarzian, Ali.

In: Alcoholism: Clinical and Experimental Research, Vol. 43, No. 9, 01.09.2019, p. 1898-1908.

Research output: Contribution to journalArticle

Bishehsari, F, Zhang, L, Voigt, RM, Maltby, N, Semsarieh, B, Zorub, E, Shaikh, M, Wilber, S, Armstrong, AR, Mirbagheri, SS, Preite, NZ, Song, P, Stornetta, A, Balbo, S, Forsyth, CB & Keshavarzian, A 2019, 'Alcohol Effects on Colon Epithelium are Time-Dependent', Alcoholism: Clinical and Experimental Research, vol. 43, no. 9, pp. 1898-1908. https://doi.org/10.1111/acer.14141
Bishehsari F, Zhang L, Voigt RM, Maltby N, Semsarieh B, Zorub E et al. Alcohol Effects on Colon Epithelium are Time-Dependent. Alcoholism: Clinical and Experimental Research. 2019 Sep 1;43(9):1898-1908. https://doi.org/10.1111/acer.14141
Bishehsari, Faraz ; Zhang, Lijuan ; Voigt, Robin M. ; Maltby, Natalie ; Semsarieh, Bita ; Zorub, Eyas ; Shaikh, Maliha ; Wilber, Sherry ; Armstrong, Andrew R. ; Mirbagheri, Seyed Sina ; Preite, Nailliw Z. ; Song, Peter ; Stornetta, Alessia ; Balbo, Silvia ; Forsyth, Christopher B. ; Keshavarzian, Ali. / Alcohol Effects on Colon Epithelium are Time-Dependent. In: Alcoholism: Clinical and Experimental Research. 2019 ; Vol. 43, No. 9. pp. 1898-1908.
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abstract = "Background: Alcohol intake increases the risk of developing colon cancer. Circadian disruption promotes alcohol's effect on colon carcinogenesis through unknown mechanisms. Alcohol's metabolites induce DNA damage, an early step in carcinogenesis. We assessed the effect of time of alcohol consumption on markers of tissue damage in the colonic epithelium. Methods: Mice were treated by alcohol or phosphate-buffered saline (PBS), at 4-hour intervals for 3 days, and their colons were analyzed for (i) proliferation (Ki67) and antiapoptosis (Bcl-2) markers, (ii) DNA damage (γ-H2AX), and (iii) the major acetaldehyde (AcH)–DNA adduct, N2-ethylidene-dG. To model circadian disruption, mice were shifted once weekly for 12 h and then were sacrificed at 4-hour intervals. Samples of mice with a dysfunctional molecular clock were analyzed. The dynamics of DNA damage repair from AcH treatment as well as role of xeroderma pigmentosum, complementation group A (XPA) in their repair were studied in vitro. Results: Proliferation and survival of colonic epithelium have daily rhythmicity. Alcohol induced colonic epithelium proliferation in a time-dependent manner, with a stronger effect during the light/rest period. Alcohol-associated DNA damage also occurred more when alcohol was given at light. Levels of DNA adduct did not vary by time, suggesting rather lower repair efficiency during the light versus dark. XPA gene expression, a key excision repair gene, was time-dependent, peaking at the beginning of the dark. XPA knockout colon epithelial cells were inefficient in repair of the DNA damage induced by alcohol's metabolite. Conclusions: Time of day of alcohol intake may be an important determinant of colon tissue damage and carcinogenicity.",
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AU - Zorub, Eyas

AU - Shaikh, Maliha

AU - Wilber, Sherry

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