The transcription factor Aire controls immunological tolerance by inducing the ectopic thymic expression of many tissue-specific genes, acting broadly by removing stops on the transcriptional machinery. To better understand Aire's specificity, we performed single-cell RNA-seq and DNA-methylation analysis of Aire-sufficient and Aire-deficient medullary epithelial cells (mTECs). Each of Aire's target genes was induced in only a minority of mTECs, independently of DNA-methylation patterns, as small inter-chromosomal gene clusters activated in concert in a proportion of mTECs. These microclusters differed between individual mice. Thus, our results suggest an organization of the DNA or of the epigenome that results from stochastic determinism but is 'bookmarked' and stable through mTEC divisions, which ensures more effective presentation of self antigens and favors diversity of self-tolerance between individuals.
|Original language||English (US)|
|Number of pages||8|
|State||Published - Aug 19 2015|
Bibliographical noteFunding Information:
We thank S. Mostafavi for advice on computational analysis; M. Anderson (University of California, San Francisco) for the Aire-GFP line; and K. Hattori, G. Buruzula, and K. Waraska for help with mice, sorting and sequencing. Supported by the US National Institutes of Health (DK060027; and a Children’s Hospital in Pediatric Gastroenterology training grant for M.M.) and Boehringer Ingelheim Fonds (D.Z.).
© 2015 Nature America, Inc.
PubMed: MeSH publication types
- Journal Article
- Research Support, N.I.H., Extramural
- Research Support, Non-U.S. Gov't