Aging impairs the expression of the catalytic subunit of glutamate cysteine ligase in soleus muscle under stress

Chiao Nan Chen, Holly M. Brown-Borg, Sharlene G. Rakoczy, Deborah A. Ferrington, Ladora V. Thompson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This study investigated the mechanisms responsible for the disrupted homeostasis of reduced glutathione (GSH) in aging muscles with stress (14 days of hind-limb unloading [HU]). Adult and old rats were randomized into four groups: weight bearing and 3, 7, and 14 days of HU. Soleus muscles were harvested to investigate the activity or content of enzymes involved in GSH metabolism (utilization and synthesis). The activities of glutathione S transferase, glutathione reductase, γ-glutamyl transpeptidase, and glutamate cysteine ligase (GCL) were determined. The protein content of the two subunits of GCL, catalytic subunit (GCLC) and modifier subunit (GCLM), were evaluated. The major results, failure to maintain the accelerated GCLC production and GCL activity, are associated with the GSH depletion in aging muscles with 14 days of HU. The results suggest that the regulation of GCL, especially the catalytic subunit, with stress may be compromised in aging muscles.

Original languageEnglish (US)
Pages (from-to)129-137
Number of pages9
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume65
Issue number2
DOIs
StatePublished - Apr 9 2010

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Glutamate-Cysteine Ligase
Catalytic Domain
Skeletal Muscle
Extremities
Muscles
gamma-Glutamyltransferase
Glutathione Reductase
Weight-Bearing
Glutathione Transferase
Glutathione
Homeostasis
Enzymes
Proteins

Keywords

  • GCL
  • GCLC
  • Hind-limb unloading

Cite this

Aging impairs the expression of the catalytic subunit of glutamate cysteine ligase in soleus muscle under stress. / Chen, Chiao Nan; Brown-Borg, Holly M.; Rakoczy, Sharlene G.; Ferrington, Deborah A.; Thompson, Ladora V.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 65, No. 2, 09.04.2010, p. 129-137.

Research output: Contribution to journalArticle

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