Age, serum 25-hydroxyvitamin D and vitamin D receptor (VDR) expression and function in peripheral blood mononuclear cells

Laura A. Coleman, Margarita Mishina, Mark Thompson, Sarah M. Spencer, Adrian J. Reber, William G. Davis, Po Yung Cheng, Edward A. Belongia, H. Keipp Talbot, Maria E. Sundaram, Marie R. Griffin, David K. Shay, Suryaprakash Sambhara

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The relationship between age, vitamin D status, expression and functionality of the vitamin D receptor (VDR), and key genes in the vitamin D pathway in immune cells is unclear. We enrolled adults 50 to 69 years old (20 subjects) and 70+ (20 subjects) and measured: 1) 25(OH)D levels by liquid chromatography/mass spectrometry; and 2) mRNA expression of VDR, 1α-OHase, 1,25D3-MARRS, TREM-1, cathelicidin, RIG-I, and interferon-β by qRT-PCR. Mean serum 25(OH)D was 30 ± 4 ng/mL and was not associated with age. Baseline expression of VDR, 1α-OHase, 1,25D3-MARRS, TREM-1, and RIG-I also did not differ by age; IFN-β expression, however, was higher in the 70+ year old group. 25(OH)D3- and 1,25(OH)2D3-induced VDR, TREM-1 and cathelicidin expression were similar between age groups, as was LPS-induced expression of VDR and of 1α-OHase. Ligand-induced 1,25D3-MARRS expression was higher in subjects ≥ 70 years. Serum 25(OH)D was inversely associated with LPS-stimulated VDR expression and with baseline or vitamin D-induced TREM-1 expression, adjusting for age, self-rated health, and functional status. In healthy adults ≥ 50 years, the expression and functionality of the VDR, 1α-OHase and key vitamin D pathway genes were not consistently associated with age.

Original languageEnglish (US)
Pages (from-to)35512-35521
Number of pages10
JournalOncotarget
Volume7
Issue number24
DOIs
StatePublished - 2016

Keywords

  • 1α-hydroxylase
  • Gerotarget
  • MRNA expression
  • PBMC
  • Vitamin D
  • Vitamin D Receptor

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    Coleman, L. A., Mishina, M., Thompson, M., Spencer, S. M., Reber, A. J., Davis, W. G., Cheng, P. Y., Belongia, E. A., Talbot, H. K., Sundaram, M. E., Griffin, M. R., Shay, D. K., & Sambhara, S. (2016). Age, serum 25-hydroxyvitamin D and vitamin D receptor (VDR) expression and function in peripheral blood mononuclear cells. Oncotarget, 7(24), 35512-35521. https://doi.org/10.18632/oncotarget.9398