Age-related changes in collagen properties and mineralization in cancellous and cortical bone in the porcine mandibular condyle

Nop M.B.K. Willems, Geerling E.J. Langenbach, Vincent Everts, Lars Mulder, Thorsten Grünheid, Ruud A. Bank, Andrej Zentner, Theo M.G.J. Van Eijden

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Collagen is an important constituent of bone, and it has been suggested that changes in collagen and mineral properties of bone are interrelated during growth. The aim of this study was to quantify age-related changes in collagen properties and the degree of mineralization of bone (DMB). The DMB in cancellous and cortical bone samples from the mandibular condyle of 35 female pigs aged 0-100 weeks was determined using micro-computed tomography. Subsequently, the amount of collagen and the number of pentosidine (Pen), hydroxylysylpyridinoline (HP), and lysylpyridinoline (LP) cross-links were quantified by means of high-performance liquid chromatography. The amount of collagen increased with age in cancellous bone but remained unchanged in cortical bone. The number of Pen and LP cross-links decreased in both bone types. In contrast, the number of HP cross-links decreased only in cancellous bone. The sum of the number of HP and LP cross-links decreased with age in cancellous bone only. The DMB increased in cancellous and cortical bone. It was concluded that the largest changes in the number of mature collagen cross-links and the mineralization in porcine cancellous and cortical bone take place before the age of 40 weeks. The low number of mature cross-links after this age suggests that the bone turnover rate continues to be high and thereby prevents the development of mature cross-links.

Original languageEnglish (US)
Pages (from-to)307-312
Number of pages6
JournalCalcified Tissue International
Volume86
Issue number4
DOIs
StatePublished - Apr 2010

Keywords

  • Bone
  • Collagen
  • Mineralization
  • Pentosidine
  • Remodeling

Fingerprint Dive into the research topics of 'Age-related changes in collagen properties and mineralization in cancellous and cortical bone in the porcine mandibular condyle'. Together they form a unique fingerprint.

  • Cite this

    Willems, N. M. B. K., Langenbach, G. E. J., Everts, V., Mulder, L., Grünheid, T., Bank, R. A., Zentner, A., & Van Eijden, T. M. G. J. (2010). Age-related changes in collagen properties and mineralization in cancellous and cortical bone in the porcine mandibular condyle. Calcified Tissue International, 86(4), 307-312. https://doi.org/10.1007/s00223-010-9339-2