Age dependent effects of NELL-1 isoforms on bone marrow stromal cells

Carolyn A. Meyers; Zhibo Sun; Leslie Chang; Catherine Ding; Amy Lu; Kang Ting; Shen Pang; Aaron W. James

doi:10.1016/j.jor.2019.02.006

Age dependent effects of NELL-1 isoforms on bone marrow stromal cells

Carolyn A. Meyers, Zhibo Sun, Leslie Chang, Catherine Ding, Amy Lu, Kang Ting, Shen Pang, Aaron W. James

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Objective: NELL-1 is an osteogenic protein first discovered to control ossification of the cranium. NELL-1 exists in at least two isoforms. The full-length NELL-1 contains 810 amino acid (aa) (NELL-1810), the N-terminal-truncated NELL-1 isoform contains 570 aa (NELL-1570). The differences in cellular effects between NELL-1 isoforms are not well understood. Methods: Here, BMSC were derived from adult or aged mice, followed by overexpression of NELL-1810 or NELL-1570. Cell morphology, proliferation, and gene expression were examined. Results/Conclusions: Overall, the proliferative effect of NELL-1570 was age dependent, showing prominent induction in adult but not aged mice.

Original language	English (US)
Pages (from-to)	175-178
Number of pages	4
Journal	Journal of Orthopaedics
Volume	16
Issue number	2
DOIs	https://doi.org/10.1016/j.jor.2019.02.006
State	Published - Mar 1 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 Prof. PK Surendran Memorial Education Foundation

Keywords

BMSC
Mesenchymal stem cell
Nell-1
Osteogenesis

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10.1016/j.jor.2019.02.006

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title = "Age dependent effects of NELL-1 isoforms on bone marrow stromal cells",

abstract = "Objective: NELL-1 is an osteogenic protein first discovered to control ossification of the cranium. NELL-1 exists in at least two isoforms. The full-length NELL-1 contains 810 amino acid (aa) (NELL-1810), the N-terminal-truncated NELL-1 isoform contains 570 aa (NELL-1570). The differences in cellular effects between NELL-1 isoforms are not well understood. Methods: Here, BMSC were derived from adult or aged mice, followed by overexpression of NELL-1810 or NELL-1570. Cell morphology, proliferation, and gene expression were examined. Results/Conclusions: Overall, the proliferative effect of NELL-1570 was age dependent, showing prominent induction in adult but not aged mice.",

keywords = "BMSC, Mesenchymal stem cell, Nell-1, Osteogenesis",

author = "Meyers, {Carolyn A.} and Zhibo Sun and Leslie Chang and Catherine Ding and Amy Lu and Kang Ting and Shen Pang and James, {Aaron W.}",

note = "Publisher Copyright: {\textcopyright} 2019 Prof. PK Surendran Memorial Education Foundation",

year = "2019",

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doi = "10.1016/j.jor.2019.02.006",

language = "English (US)",

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journal = "Journal of Orthopaedics",

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TY - JOUR

T1 - Age dependent effects of NELL-1 isoforms on bone marrow stromal cells

AU - Meyers, Carolyn A.

AU - Sun, Zhibo

AU - Chang, Leslie

AU - Ding, Catherine

AU - Lu, Amy

AU - Ting, Kang

AU - Pang, Shen

AU - James, Aaron W.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Objective: NELL-1 is an osteogenic protein first discovered to control ossification of the cranium. NELL-1 exists in at least two isoforms. The full-length NELL-1 contains 810 amino acid (aa) (NELL-1810), the N-terminal-truncated NELL-1 isoform contains 570 aa (NELL-1570). The differences in cellular effects between NELL-1 isoforms are not well understood. Methods: Here, BMSC were derived from adult or aged mice, followed by overexpression of NELL-1810 or NELL-1570. Cell morphology, proliferation, and gene expression were examined. Results/Conclusions: Overall, the proliferative effect of NELL-1570 was age dependent, showing prominent induction in adult but not aged mice.

AB - Objective: NELL-1 is an osteogenic protein first discovered to control ossification of the cranium. NELL-1 exists in at least two isoforms. The full-length NELL-1 contains 810 amino acid (aa) (NELL-1810), the N-terminal-truncated NELL-1 isoform contains 570 aa (NELL-1570). The differences in cellular effects between NELL-1 isoforms are not well understood. Methods: Here, BMSC were derived from adult or aged mice, followed by overexpression of NELL-1810 or NELL-1570. Cell morphology, proliferation, and gene expression were examined. Results/Conclusions: Overall, the proliferative effect of NELL-1570 was age dependent, showing prominent induction in adult but not aged mice.

KW - BMSC

KW - Mesenchymal stem cell

KW - Nell-1

KW - Osteogenesis

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Age dependent effects of NELL-1 isoforms on bone marrow stromal cells

Abstract

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Keywords

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