African-lineage zika virus replication dynamics and maternal-fetal interface infection in pregnant rhesus macaques

Chelsea M. Crooks, Andrea M. Weiler, Sierra L. Rybarczyk, Mason Bliss, Anna S. Jaeger, Megan E. Murphy, Heather A. Simmons, Andres Mejia, Michael K. Fritsch, Jennifer M. Hayes, Jens C. Eickhoff, Ann M. Mitzey, Elaina Razo, Katarina M. Braun, Elizabeth A. Brown, Keisuke Yamamoto, Phoenix M. Shepherd, Amber Possell, Kara Weaver, Kathleen M. AntonyTerry K. Morgan, Xiankun Zeng, Dawn M. Dudley, Eric Peterson, Nancy Schultz-Darken, David H. O’Connor, Emma L. Mohr, Thaddeus G. Golos, Matthew T. Aliota, Thomas C. Friedrich

Research output: Contribution to journalArticlepeer-review

Abstract

Following the Zika virus (ZIKV) outbreak in the Americas, ZIKV was causally associated with microcephaly and a range of neurological and developmental symptoms, termed congenital Zika syndrome (CZS). The viruses responsible for this outbreak belonged to the Asian lineage of ZIKV. However, in vitro and in vivo studies assessing the pathogenesis of African-lineage ZIKV demonstrated that African-lineage isolates often replicated to high titers and caused more-severe pathology than Asian-lineage isolates. To date, the pathogenesis of African-lineage ZIKV in a translational model, particularly during pregnancy, has not been rigorously characterized. Here, we infected four pregnant rhesus macaques with a low-passage-number strain of African-lineage ZIKV and compared its pathogenesis to those for a cohort of four pregnant rhesus macaques infected with an Asian-lineage isolate and a cohort of mock-inoculated controls. The viral replication kinetics for the two experimental groups were not significantly different, and both groups developed robust neutralizing antibody titers above levels considered to be protective. There was no evidence of significant fetal head growth restriction or gross fetal harm at delivery (1 to 1.5 weeks prior to full term) in either group. However, a significantly higher burden of ZIKV viral RNA (vRNA) was found in the maternal-fetal interface tissues of the macaques exposed to an African-lineage isolate. Our findings suggest that ZIKV of any genetic lineage poses a threat to pregnant individuals and their infants. IMPORTANCE ZIKV was first identified in 1947 in Africa, but most of our knowledge of ZIKV is based on studies of the distinct Asian genetic lineage, which caused the outbreak in the Americas in 2015 to 2016. In its most recent update, the WHO stated that improved understanding of African-lineage ZIKV pathogenesis during pregnancy must be a priority. The recent detection of African-lineage isolates in Brazil underscores the need to understand the impact of these viruses. Here, we provide the first comprehensive assessment of African-lineage ZIKV infection during pregnancy in a translational nonhuman primate model. We show that African-lineage isolates replicate with kinetics similar to those of Asian-lineage isolates and can infect the placenta. However, there was no evidence of more-severe outcomes with African-lineage isolates. Our results highlight both the threat that African-lineage ZIKV poses to pregnant individuals and their infants and the need for epidemiological and translational in vivo studies with African-lineage ZIKV.

Original languageEnglish (US)
Article numbere02220
JournalJournal of virology
Volume95
Issue number16
DOIs
StatePublished - Aug 2021

Bibliographical note

Funding Information:
This work was supported by award R01AI132563 from the National Institute of Allergy and Infectious Diseases and award P51OD011106 from the Office of the Director, NIH, to the Wisconsin National Primate Research Center. Chelsea M. Crooks was supported by award T32 AI007414 from the National Institute of Allergy and Infectious Diseases.

Publisher Copyright:
Copyright © 2021 Crooks et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

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