AFLP Fingerprinting and Genetic Diversity of Main Sweetpotato Varieties in China

De Gao Liu, Ning Zhao, Hong Zhai, Xiao Xia Yu, Qin Jie, Lian Jun Wang, Shao Zhen He, Qing Chang Liu

Research output: Contribution to journalArticlepeer-review

Abstract

AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta. /Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance (AMOVA) revealed greater variations within regions (94.08%) than among regions (5.92%). Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between " Northern" and " Southern" sweetpotato varieties when Yangtze River was used as the dividing line.

Original languageEnglish (US)
Pages (from-to)1424-1433
Number of pages10
JournalJournal of Integrative Agriculture
Volume11
Issue number9
DOIs
StatePublished - Sep 2012
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by China Agriculture Research System (Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008).

Keywords

  • AFLP fingerprinting
  • Genetic diversity
  • Main variety
  • Sweetpotato (Ipomoea batatas (L.) Lam.)

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