Aerotolerance and multilocus sequence typing among Campylobacter jejuni strains isolated from humans, broiler chickens, and cattle in Miyazaki Prefecture, Japan

Savek Kiatsomphob, Takako Taniguchi, Elpita Tarigan, Khin Maung Latt, Byeonghwa Jeon, Naoaki Misawa

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Campylobacter jejuni is one of the leading causes of human gastroenteritis in Japan. As chickens and cattle are common reservoirs for C. jejuni, this microaerophilic, stress-sensitive bacterium can overcome and survive various stress conditions during zoonotic transmission, particularly foodborne, to humans. How C. jejuni overcomes stress conditions is, however, unclear. In the present study, 70 C. jejuni strains isolated from various sources (26 human, 20 broilers, and 24 cattle isolates) in Miyazaki, Japan, from 2010 to 2012, were subjected to multilocus sequence typing (MLST) and aerotolerance testing (aerobic shaking at 200 rpm). The results demonstrated that C. jejuni strains from Miyazaki belonged to 12 clonal complexes (CCs) and 43 sequence types (STs). CC-21 and CC-460 were mainly detected in human clinical strains. Most tested strains were aerotolerant, and only one (1.4%) was deemed sensitive to aerobic stress. Approximately 40% strains survived the 24-hr vigorous aerobic shaking at 200 rpm, and these hyper-aerotolerant strains were more prevalent in broiler and cattle isolates than in human isolates. Phylogenetic analysis divided the strains into five clusters, each showing a different pattern of host association. Thus, we have demonstrated for the first time that C. jejuni strains with increased tolerance to aerobic stress are highly prevalent in broilers and cattle in Miyazaki, Japan, and that certain clonal populations are frequently implicated in human infection in this area.

Original languageEnglish (US)
Pages (from-to)1144-1151
Number of pages8
JournalJournal of Veterinary Medical Science
Volume81
Issue number8
DOIs
StatePublished - 2019
Externally publishedYes

Bibliographical note

Funding Information:
ACKNOWLEDGMENT. The work was supported by grants for a project on Core-to-Core Program, Asia-Africa Science Platforms by the Japan Society for the Promotion of Science (JSPS).

Publisher Copyright:
© 2019 The Japanese Society of Veterinary Science.

Keywords

  • Aerotolerance
  • Campylobacter jejuni
  • Japan isolate
  • Multilocus sequence typing (MLST)

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